Faf2 (NM_178397) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG207077
Faf2 (tGFP-tagged) - Mouse UBX domain containing 8 (Ubxd8)
  $657.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Faf2
Synonyms 2210404D11Rik; AI462440; mKIAA0887; Ubxd8
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG207077 representing NM_178397
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCGCCTGAGGAGCAGGATCTAACTCAGGAGCAGACCGAGAAGCTGCTGCAGTTTCAGGATCTGA
CTGGCATAGAGTCCATGGAGCAGTGTCGCCTTGCTTTGGAGCAGCATAACTGGAACATGGAGGCTGCTGT
CCAGGACAGACTGAATGAGCAGGAAGGTGTACCAAGTGTTTTCAATCCACCGCCAGCTCGGCCCCTACAG
GTTACAGCTGACCACAGGATCTACAGCTATGTTGTCTCAAGACCACAACCAAGGGGCCTGCTTGGATGGG
GTTATTACTTGATAATGCTTCCATTCCGGTTTACCTATTACACGATACTTGATATATTTAGGTTTGCTCT
TCGTTTTATACGGCCTGACCCTCGCAGCCGGGTCACTGACCCTGTTGGGGACATTGTTTCATTTATGCAC
TCTTTTGAAGAGAAATATGGGAGGGCACACCCTGTCTTCTACCAGGGAACGTACAGCCAGGCACTTAACG
ATGCCAAGCGGGAACTTCGATTTCTTTTGGTTTATCTTCATGGAGATGACCACCAGGACTCTGATGAGTT
CTGCCGCAATGCTCTCTGCGCACCTGAGGTCATCTCACTGATAAACAGCAGGATGCTTTTCTGGGCTTGT
TCTACAAACAAGCCTGAGGGGTACAGGGTGTCCCAGGCCTTACGGGAGAACACCTATCCATTCCTAGCTA
TGATTATGTTGAAGGATCGGCGAATGACTGTGGTTGGAAGGCTAGAAGGCCTCATTCAACCTGATGACCT
CATCAACCAGCTGACATTCATCATGGATGCAAACCAGACTTACCTGGTGTCAGAACGTCTAGAAAGGGAA
GAAAGGAACCAAACCCAGGTGCTGAGACAACAGCAGGATGAAGCCTACCTGGCTTCTCTCAGGGCAGATC
AGGAGAAAGAGAGGAAGAAGAGGGAGGAGAAAGAGCGGAAACGCCGGAAGGAGGAGGAGGTGCAGCAACA
GAAGCTGGCGGAGGAGCGAAGACGACAGAACTTGCAGGAGGAAAAGGAGAGGAAGCTGGAATGCCTGCCC
CCTGAGCCCTCCCCTGATGATCCTGAAAGTGTCAAGATCATTTTCAAGTTACCCAATGATTCCAGAGTAG
AGAGACGATTCCACTTCTCACAGTCTCTAACAGTCATCCATGACTTCTTATTCTCCTTGAAGGAAAGCCC
TGAAAAGTTTCAGATTGAAGCCAATTTTCCAAGACGGGTACTTCCCTGTGTTCCTTCAGAGGAATGGCCC
AACCCCCCAACACTGCAGGAGGCGGGACTCAGCCACACAGAAGTTCTCTTTGTCCAGGACCTGACAGACG
AA


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG207077 representing NM_178397
Red=Cloning site Green=Tags(s)

MAAPEEQDLTQEQTEKLLQFQDLTGIESMEQCRLALEQHNWNMEAAVQDRLNEQEGVPSVFNPPPARPLQ
VTADHRIYSYVVSRPQPRGLLGWGYYLIMLPFRFTYYTILDIFRFALRFIRPDPRSRVTDPVGDIVSFMH
SFEEKYGRAHPVFYQGTYSQALNDAKRELRFLLVYLHGDDHQDSDEFCRNALCAPEVISLINSRMLFWAC
STNKPEGYRVSQALRENTYPFLAMIMLKDRRMTVVGRLEGLIQPDDLINQLTFIMDANQTYLVSERLERE
ERNQTQVLRQQQDEAYLASLRADQEKERKKREEKERKRRKEEEVQQQKLAEERRRQNLQEEKERKLECLP
PEPSPDDPESVKIIFKLPNDSRVERRFHFSQSLTVIHDFLFSLKESPEKFQIEANFPRRVLPCVPSEEWP
NPPTLQEAGLSHTEVLFVQDLTDE

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_178397
ORF Size 1332 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_178397.2, NP_848484.1
RefSeq Size 4304 bp
RefSeq ORF 1338 bp
Locus ID 76577
UniProt ID Q3TDN2
Cytogenetics 13 B1
Summary Plays an important role in endoplasmic reticulum-associated degradation (ERAD) that mediates ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins. By controlling the steady-state expression of the IGF1R receptor, indirectly regulates the insulin-like growth factor receptor signaling pathway. Involved in inhibition of lipid droplet degradation by binding to phospholipase PNPL2 and inhibiting its activity by promoting dissociation of PNPL2 from its endogenous activator, ABHD5 which inhibits the rate of triacylglycerol hydrolysis.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_178397" in other vectors (4)
SKU Description Size Price
MC215907 Faf2 (untagged) - Mouse Fas associated factor family member 2 (Faf2), (10ug) 10 ug
$503.00
MR207077 Faf2 (Myc-DDK-tagged) - Mouse Fas associated factor family member 2 (Faf2) 10 ug
$289.00 $457.00
MR207077L3 Lenti ORF clone of Faf2 (Myc-DDK-tagged) - Mouse Fas associated factor family member 2 (Faf2) 10 ug
$757.00
MR207077L4 Lenti ORF clone of Faf2 (mGFP-tagged) - Mouse Fas associated factor family member 2 (Faf2) 10 ug
$757.00

Plasmid Map for MG207077

Download sequence for

Cloning scheme for MG207077

Circular map for MG207077

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