Zmynd10 (NM_053253) Mouse Tagged ORF Clone

SKU
MG207003
Zmynd10 (tGFP-tagged) - Mouse zinc finger, MYND domain containing 10 (Zmynd10)
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    TrueORF®

    Expression-ready ORF plasmid with C-terminal tag(s)

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$489.00 MSRP $657.00 MSRP $657.00
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Specifications
Product Data
Target Symbol Zmynd10
Synonyms Blu
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG207003 representing NM_053253
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGTGACCTGGAACTGCTGCTGCCTGGGGAGGCTGAGGTGCTGGTGCGGGGCCTGCGCAGCTTCCAGC
TTCGGGAGATGGGCTCTGAAGGGTGGAATAAGCAGCATGAGAGCCTGGAGAAGCTGAACATGCAAGCCAT
CCTTGATGCAACTATCAGCCAGGCTGAGCCCATCCAGGAACTGCTAATCAACCATGGGAAGATCCCAACG
CTGGTGGAGGAGCTGATTGCGGTGGAGATGTGGAAACAGAAGGTTTTCCCTGTGCTGTGCCGGCTGGAGG
ACTTCAAGCCCCAGAATACCTTCCCCATATACATGGTGGTACACCATGAGGCCTCCATCATCAATCTCCT
AGAGACAGTGTTCTTCCACAAGGAGGTGTGTGAGTCAGCTGATGACAAGGTCTTGGACCTAGTAGACTAT
TGCCACCGCAAGCTGATACTGCTAGTGGCCCGGAAAGGCGGTGGTGACCTGTCTGAAGAAGAACAGTTCC
AGGACAGTACCCCTATGCAAGAGCTGCAAAAGCAGGCGGAGATGATGGAATTTGAGATATCCCTGAAAGC
CCTCTCGGTGCTTCGCTACATCACAGACTGCGTGGATAGCCTTTCCCTGAGCACACTGAACCGCATGCTC
AGGACTCACAACTTGCCCTGCCTCTTGGTGGAACTGCTGGAGCACAGTCCCTGGAGCCGGCGGGTAGGAG
GCAAGCTGCAGCATTTTGAGAGTGGCCGATGGCAGACGGTGGCCCCCTCAGAGCAGCAAAAGCTGAATAA
ACTGGATGGGCAAGTATGGATCGCCCTGTACAATCTACTGCTCAGCCCTGAGGCCCGAGCCCGTTACTGC
CTTACAAGCTTTGCCAAGGGACAGCTGCTTAAGCTTCAGGCCTTCCTCACTGACACACTACTCGACCAGT
TGCCCAATCTTGCCGATCTGAAGGGTTTCCTGGCCCACCTGTCCCTGGCTGAAACCCAGCCCCCTAAGAA
GGACCTAGTGTTAGAACAGATCCCAGAAATCTGGGATCGCCTGGAGAGAGAGAACAAAGGGAAATGGCAG
GCTATCGCCAAGCACCAGCTTCAGCACGTATTCAGCCTCTCGGAGAAGGATCTTCGTCAACAAGCACAGA
GGTGGGCTGAAACCTACAGGCTGGATGTCCTAGAGGCAGTAGCTCCGGAGAGGCCCCGCTGCGGCTACTG
CAACGCAGAGGCCTCCAAGCGCTGCTCCAGATGCCAGAATGTGTGGTATTGCTGCAGGGAGTGTCAAGTC
AAGCACTGGGAGAAGCACGGAAAGACATGTGTTCTAGCAGCCCAAGGTGACAGAGCCAAG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG207003 representing NM_053253
Red=Cloning site Green=Tags(s)

MGDLELLLPGEAEVLVRGLRSFQLREMGSEGWNKQHESLEKLNMQAILDATISQAEPIQELLINHGKIPT
LVEELIAVEMWKQKVFPVLCRLEDFKPQNTFPIYMVVHHEASIINLLETVFFHKEVCESADDKVLDLVDY
CHRKLILLVARKGGGDLSEEEQFQDSTPMQELQKQAEMMEFEISLKALSVLRYITDCVDSLSLSTLNRML
RTHNLPCLLVELLEHSPWSRRVGGKLQHFESGRWQTVAPSEQQKLNKLDGQVWIALYNLLLSPEARARYC
LTSFAKGQLLKLQAFLTDTLLDQLPNLADLKGFLAHLSLAETQPPKKDLVLEQIPEIWDRLERENKGKWQ
AIAKHQLQHVFSLSEKDLRQQAQRWAETYRLDVLEAVAPERPRCGYCNAEASKRCSRCQNVWYCCRECQV
KHWEKHGKTCVLAAQGDRAK

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN NM_053253
ORF Size 1320 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_053253.4
RefSeq Size 1784 bp
RefSeq ORF 1323 bp
Locus ID 114602
UniProt ID Q99ML0
Cytogenetics 9 F1
Summary Plays a role in axonemal structure organization and motility (PubMed:29601588). Involved in axonemal pre-assembly of inner and outer dynein arms (IDA and ODA, respectively) for proper axoneme building for cilia motility (PubMed:29601588). May act by indirectly regulating transcription of dynein proteins (By similarity).[UniProtKB/Swiss-Prot Function]
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SKU Description Size Price
MC215859 Zmynd10 (untagged) - Mouse zinc finger, MYND domain containing 10 (Zmynd10), (10ug) 10 ug
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MR207003 Zmynd10 (Myc-DDK-tagged) - Mouse zinc finger, MYND domain containing 10 (Zmynd10) 10 ug
$289.00 MSRP $457.00 MSRP $457.00
MR207003L3 Lenti ORF clone of Zmynd10 (Myc-DDK-tagged) - Mouse zinc finger, MYND domain containing 10 (Zmynd10) 10 ug
$757.00
MR207003L4 Lenti ORF clone of Zmynd10 (mGFP-tagged) - Mouse zinc finger, MYND domain containing 10 (Zmynd10) 10 ug
$757.00
Citations
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