Eno2 (NM_013509) Mouse Tagged ORF Clone

CAT#: MG206917

Eno2 (tGFP-tagged) - Mouse enolase 2, gamma neuronal (Eno2)

Product Images

Circular map for MG206917

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Eno2
• Synonyms: AI837106; D6Ertd375e; Eno-2; NSE
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG206917 representing NM_013509
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTATAGAGAAGATTTGGGCCCGAGAGATCTTGGACTCCCGTGGGAATCCCACCGTGGAGGTGGATC
  TCTATACTGCCAAAGGTCTTTTCCGGGCTGCAGTCCCCAGTGGAGCCTCCACCGGCATCTATGAGGCCCT
  GGAACTAAGGGATGGGGACAAACAGCGTTACTTAGGCAAAGGTGTCCTGAAGGCAGTGGACCACATCAAC
  AGCAGGATTGCACCAGCCCTCATCAGCTCAGGTATCTCCGTGGTGGAGCAGGAGAAACTGGACAACCTGA
  TGTTGGAACTGGATGGGACTGAGAATAAATCCAAGTTTGGGGCCAATGCCATCCTGGGTGTGTCCCTGGC
  CGTGTGTAAGGCTGGGGCAGCTGAGAGGGACTTGCCCCTCTATCGCCACATTGCTCAGCTAGCTGGGAAC
  TCCGACCTCATCCTGCCTGTGCCGGCCTTTAATGTGATCAATGGTGGCTCTCATGCTGGGAACAAGTTGG
  CCATGCAGGAGTTCATGATCCTCCCAGTGGGTGCTGAGAGCTTTCGGGATGCCATGCGACTTGGGGCAGA
  GGTGTACCACACCCTCAAGGGGGTCATCAAGGACAAGTATGGCAAGGATGCCACTAACGTGGGGGATGAA
  GGCGGCTTTGCCCCCAATATCCTGGAGAACAGCGAAGCCTTGGAGCTGGTGAAGGAAGCCATCGACAAGG
  CTGGCTACACGGAAAAGATGGTGATCGGTATGGATGTGGCTGCCTCTGAGTTTTACCGCGATGGCAAATA
  CGACTTGGATTTCAAGTCTCCCGCTGATCCTTCCCGATACATCACTGGGGACCAGCTGGGGGCACTCTAC
  CAGGACTTTGTCCGGAACTATCCTGTGGTCTCCATTGAAGACCCATTTGACCAGGACGACTGGGCAGCTT
  GGTCCAAGTTCACAGCCAACGTCGGCATCCAGATAGTGGGCGATGACCTGACGGTGACCAACCCCAAGCG
  CATCGAGCGGGCAGTGGAGGAGAAGGCCTGCAACTGTTTGCTGCTCAAGGTCAATCAGATCGGCTCGGTC
  ACAGAAGCCATCCAAGCGTGCAAGCTGGCCCAGGAGAATGGCTGGGGGGTTATGGTGAGCCATCGCTCTG
  GAGAAACGGAGGACACGTTCATTGCTGATCTTGTCGTCGGACTGTGTACAGGCCAGATCAAGACTGGCGC
  CCCATGCAGATCTGAACGTCTGGCGAAGTACAACCAGCTCATGAGAATTGAGGAAGAGCTGGGGGACGAA
  GCTCGCTTCGCGGGACATAATTTCCGAAATCCCAGTGTGCTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG206917 representing NM_013509
  Red=Cloning site Green=Tags(s)
  
  MSIEKIWAREILDSRGNPTVEVDLYTAKGLFRAAVPSGASTGIYEALELRDGDKQRYLGKGVLKAVDHIN
  SRIAPALISSGISVVEQEKLDNLMLELDGTENKSKFGANAILGVSLAVCKAGAAERDLPLYRHIAQLAGN
  SDLILPVPAFNVINGGSHAGNKLAMQEFMILPVGAESFRDAMRLGAEVYHTLKGVIKDKYGKDATNVGDE
  GGFAPNILENSEALELVKEAIDKAGYTEKMVIGMDVAASEFYRDGKYDLDFKSPADPSRYITGDQLGALY
  QDFVRNYPVVSIEDPFDQDDWAAWSKFTANVGIQIVGDDLTVTNPKRIERAVEEKACNCLLLKVNQIGSV
  TEAIQACKLAQENGWGVMVSHRSGETEDTFIADLVVGLCTGQIKTGAPCRSERLAKYNQLMRIEEELGDE
  ARFAGHNFRNPSVL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013509
• ORF Size: 1302 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013509.3
• RefSeq Size: 2656 bp
• RefSeq ORF: 1305 bp
• Locus ID: 13807
• UniProt ID: P17183
• Cytogenetics: 6 59.17 cM
• Gene Summary: Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival (By similarity).[UniProtKB/Swiss-Prot Function]