Arl13b (NM_026577) Mouse Tagged ORF Clone

CAT#: MG206808

Arl13b (tGFP-tagged) - Mouse ADP-ribosylation factor-like 13B (Arl13b)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Arl13b
• Synonyms: A530097K21Rik; A930014M17Rik; Arl2l1; C530009C10Rik; hnn
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG206808 representing NM_026577
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCAGTCTGATGGCCAACTGCTGCAACTTGTTCAAGCGGTGGCGGGAGCCTGTCAGAAAGGTGACAC
  TTGTGATGGTCGGCCTTGATAATGCTGGTAAAACAGCCACAGCAAAGGGAATCCAAGGAGAGCATCCTGA
  AGACGTAGCTCCCACTGTTGGCTTTTCTAAAATTGATCTGAGACAAGGAAAGTTCCAAGTTACCATCTTT
  GACTTAGGAGGTGGAAAAAGAATTCGGGGAATTTGGAAGAATTATTATGCTGAATCCTATGGGGTAATAT
  TTGTTGTGGATTCCAGTGATGAGGAGAGAATGGAAGAAACAAAGGAGACAATGTCCGAAGTGCTAAGACA
  CCCGAGGATATCAGGAAAGCCTATATTGGTGCTGGCCAACAAGCAAGACAAGGAGGGGGCTTTAGGCGAA
  GCGGATGTGATTGAGTGTCTCTCGCTGGAGAAGCTGGTCAACGAGCACAAGTGCCTGTGTCAGATCGAAC
  CTTGTTCAGCAGTCTTGGGATATGGAAAGAAAATTGACAAGTCCATTAAAAAGGGGCTTTATTGGCTACT
  GCATATCATTGCAAAGGACTTTGATGCCTTAAGTGAACGCATCCAGAAAGACACAACTGAACAGCGAGCT
  CTTGAGGAACAAGAGAAACGTGAGAGGGCTGAACGAGTCCGGAAGTTAAGGGAAGAAAGAGAACGAGAGC
  AGACGGAACTTGATGGGACCAGTGGTCTGGCTGAGATTGACTCAGGACCAGTTCTTGCGAATCCTTTCCA
  ACCCATTGCAGCTGTAATCATTGAGAATGAAAAGAAGCAGGAAAAAGAGAAAAAGAAGCAGACCGTGGAG
  AAGGACAGTGATGTTGGTCTCCTAGAACATAAAGTGGAGCCAGAGCAAGCAGCGCCTCAGAGTGAGGCAG
  ATTGCTGCCTCCAAAACCCTGACGAGCGAGTGGTGGACAGTTACAGGGAGGCACTGTCGCAGCAGCTGGA
  CAGTGAGGATGAGCAGGACCAGCGAGGGTCAGAGTCCGGGGAAAACAGTAAGAAGAAAACCAAGAAACTA
  AGAATGAAAAGGAGTCAGCGGGTAGAACCAGTCAATACAGACGAGTCTACTCCAAAGAGTCCCACGCCTC
  CCCAACCTCCCCCTCCTGTTGGCTGGGGAACCCCCAAAGTCACTAGACTTCCAAAACTTGAGCCTCTTGG
  TGAAACACGTCACAATGATTTCTATGGGAAGCCGCTGCCTCCCCTGGCTGTGCGACAGAGACCTAACGGT
  GATGCTCAGGACACGATCTCA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG206808 representing NM_026577
  Red=Cloning site Green=Tags(s)
  
  MFSLMANCCNLFKRWREPVRKVTLVMVGLDNAGKTATAKGIQGEHPEDVAPTVGFSKIDLRQGKFQVTIF
  DLGGGKRIRGIWKNYYAESYGVIFVVDSSDEERMEETKETMSEVLRHPRISGKPILVLANKQDKEGALGE
  ADVIECLSLEKLVNEHKCLCQIEPCSAVLGYGKKIDKSIKKGLYWLLHIIAKDFDALSERIQKDTTEQRA
  LEEQEKRERAERVRKLREEREREQTELDGTSGLAEIDSGPVLANPFQPIAAVIIENEKKQEKEKKKQTVE
  KDSDVGLLEHKVEPEQAAPQSEADCCLQNPDERVVDSYREALSQQLDSEDEQDQRGSESGENSKKKTKKL
  RMKRSQRVEPVNTDESTPKSPTPPQPPPPVGWGTPKVTRLPKLEPLGETRHNDFYGKPLPPLAVRQRPNG
  DAQDTIS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_026577
• ORF Size: 1281 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_026577.2, NP_080853.2
• RefSeq Size: 3541 bp
• RefSeq ORF: 1284 bp
• Locus ID: 68146
• UniProt ID: Q640N2
• Cytogenetics: 16 C1.3
• Gene Summary: Cilium-specific protein required to control the microtubule-based, ciliary axoneme structure. May act by maintaining the association between IFT subcomplexes A and B. Binds GTP but is not able to hydrolyze it; the GTPase activity remains unclear. Required to pattern the neural tube. Involved in cerebral cortex development: required for the initial formation of a polarized radial glial scaffold, the first step in the construction of the cerebral cortex, by regulating ciliary signaling (PubMed:23817546). Regulates the migration and placement of postmitotic interneurons in the developing cerebral cortex (PubMed:23153492).[UniProtKB/Swiss-Prot Function]