Stk25 (NM_021537) Mouse Tagged ORF Clone

CAT#: MG206799

Stk25 (tGFP-tagged) - Mouse serine/threonine kinase 25 (yeast) (Stk25)

Product Images

Circular map for MG206799

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Stk25
• Synonyms: 1500019J11Rik; AU018434; C86992; Ste20-like; Ysk1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG206799 representing NM_021537
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCACCTCCGGGGCTTCGCGCACCAGCATTCTCGTGTGGACCCAGAGGAACTCTTCACCAAGCTTG
  ACCGCATTGGCAAAGGCTCATTTGGGGAGGTGTACAAGGGGATCGACAACCACACCAAGGAAGTGGTGGC
  CATCAAGATCATTGACTTGGAAGAGGCCGAGGATGAAATTGAGGACATCCAACAGGAGATCACTGTGCTC
  AGCCAATGTGACAGTCCTTATATCACCCGCTACTTCGGCTCCTACCTGAAGAGCACCAAGCTGTGGATTA
  TCATGGAGTACCTGGGTGGTGGCTCTGCACTGGACTTGCTGAAACCTGGTCCCCTGGAAGAGACCTATAT
  TGCCACCATCTTGCGGGAGATCCTGAAAGGCCTGGATTATCTGCACTCAGAGCGCAAGATCCACCGAGAT
  ATCAAAGCTGCCAACGTGCTGCTCTCGGAGCAGGGTGATGTGAAGCTGGCAGACTTCGGTGTGGCTGGGC
  AGCTCACAGACACACAAATCAAGAGGAACACATTTGTGGGTACACCCTTCTGGATGGCACCTGAGGTTAT
  CAAGCAGTCGGCCTATGACTTCAAGGCTGACATCTGGTCCCTGGGCATCACAGCCATTGAGCTGGCCAAG
  GGGGAGCCGCCAAACTCTGATCTCCACCCTATGCGAGTCCTGTTCCTGATTCCCAAGAACAACCCACCCA
  CATTGGAGGGCCACCACAGCAAACCCTTCAAGGAGTTTGTAGAGGCCTGCCTCAATAAGGACCCACGATT
  CCGACCCACTGCCAAGGAGCTCCTGAAGCACAAATTCATCACACGATACACCAAGAAGACCTCCTTTCTC
  ACTGAACTTATTGACCGATATAAGCGCTGGAAGTCTGAGGGGCATGGAGAGGAGTCCAGTTCTGAGGATT
  CTGACATTGATGGCGAGGCTGAGGATGGGGAGCAGGGTCCCATCTGGACGTTTCCTCCAACCATCCGGCC
  AAGTCCACATAGCAAGCTGCACAAGGGAACTGCTCTGCACAGCTCACAGAAGCCTGCAGAGCCTATCAAG
  AGACAGCCAAGGTCCCAGTGCTTGTCCACACTAGTCCGTCCTGTCTTTGGAGAGCTCAAAGAGAAGCACA
  AGCAGAGCGGTGGGAGCGTGGGTGCCCTGGAGGAGCTAGAGAATGCCTTCAGCCTGGCTGAGGAATCCTG
  TCCTGGCATCTCAGACAAGCTGATGGTGCACCTGGTGGAGCGTGTGCAGAGGTTCTCACACAGCAGGAAC
  CACCTGACATCTACCCGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG206799 representing NM_021537
  Red=Cloning site Green=Tags(s)
  
  MAHLRGFAHQHSRVDPEELFTKLDRIGKGSFGEVYKGIDNHTKEVVAIKIIDLEEAEDEIEDIQQEITVL
  SQCDSPYITRYFGSYLKSTKLWIIMEYLGGGSALDLLKPGPLEETYIATILREILKGLDYLHSERKIHRD
  IKAANVLLSEQGDVKLADFGVAGQLTDTQIKRNTFVGTPFWMAPEVIKQSAYDFKADIWSLGITAIELAK
  GEPPNSDLHPMRVLFLIPKNNPPTLEGHHSKPFKEFVEACLNKDPRFRPTAKELLKHKFITRYTKKTSFL
  TELIDRYKRWKSEGHGEESSSEDSDIDGEAEDGEQGPIWTFPPTIRPSPHSKLHKGTALHSSQKPAEPIK
  RQPRSQCLSTLVRPVFGELKEKHKQSGGSVGALEELENAFSLAEESCPGISDKLMVHLVERVQRFSHSRN
  HLTSTR
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021537
• ORF Size: 1278 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021537.3, NP_067512.3
• RefSeq Size: 3254 bp
• RefSeq ORF: 1281 bp
• Locus ID: 59041
• UniProt ID: Q9Z2W1
• Cytogenetics: 1 47.25 cM
• Gene Summary: Oxidant stress-activated serine/threonine kinase that may play a role in the response to environmental stress. Targets to the Golgi apparatus where it appears to regulate protein transport events, cell adhesion, and polarity complexes important for cell migration (By similarity).[UniProtKB/Swiss-Prot Function]