Aadat (NM_011834) Mouse Tagged ORF Clone

CAT#: MG206782

  • TrueORF®

Aadat (tGFP-tagged) - Mouse aminoadipate aminotransferase (Aadat)

ORF Plasmid: DDK tGFP

Lentiviral Particles: DDK DDK w/ Puro mGFP mGFP w/ Puro


  "NM_011834" in other vectors (6)

Reconstitution Protocol

USD 886.00

In Stock*

Size
    • 10 ug

Product Images

Frequently bought together (3)
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Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Symbol Aadat
Synonyms Aadt; AI875679; Kat2; KATII; Kyat2; mKat-2
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG206782 representing NM_011834
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAATTACTCACGGTTCCTCACTGCAACGAGCCTGGCCAGAAAGCCATCTCCCATCAGAACTACAGCGG
ACATACTGAGCAAAGCACCAAAAACCCTCATCTCCCTGGCTCCTGGATCTCCAAACCCGAGCATGTTCCC
CTTTAAGTCAGCTGCCTTCACTGTGGAAAACGGAAGCACCATCCGGTTTGAAGACGACTTGATCAAAAGG
GCCCTCCAATACTCTCCAAGCTATGGAATTCCAGAACTTCTGTCCTGGCTAAAACAGTTTCAAGTAAAAT
TGCATAATCCCCCCACTGTCAACTACCCACCCAATCAAGGACAGATGGATCTCTGCATCACATCTGGCTG
CCAAGATGGTCTCTGTAAGGCATTTGAAATGCTCATCAATCCTGGAGACACTATCCTGGTCAATGAACCA
CTGTTTCCAGGAACCCTTTATGCTATGAAACCACTAGGCTGCAACATTATTAATGTCCCCAGTGATGAGC
ATGGGATTATTCCAGAGGGTCTCAAAAAAATCCTTTCCCAGTGGAAACCAGAAGATTCCAAGGATCCCAC
AAAAAAGACTCCAAAATTTCTGTATACTGTCCCAAATGGCAACAACCCTACAGGCAACTCGTTGACAGGT
GACCGCAAGAAGGAAATCTATGAGCTTGCAAGAAAATATGATTTCCTCATAATAGAAGATGACCCTTACT
ATTTTCTTCAGTTTAGCAAGCCTTGGGAGCCAACTTTTCTCTCCATGGATGTTGATGGGAGAGTCATCAG
AGCTGACACCTTTTCAAAAACCGTCTCCTCAGGGTTGAGAGTAGGGTTTATGACTGGCCCTAAGACCTTG
ATACAGAATATTGTTCTCCACACACAAGTCTCATCAGTACATGCCTGTACTCTCTCACAGCTCATGATAC
TACAGCTTCTACACCAATGGGGAGAAGAGGGTTTCCTGGCTCATATTGACAGAACTATTGATTTCTACAA
GAACCAGAGGGATTCCATATTGGCAGCTGCAGACAAGTGGTTACGTGGCTTGGCAGAGTGGCATGTTCCC
AAAGCTGGCATGTTTCTATGGATTAAAGTTAAGGGAATCTCCGATACAAAACAACTGATTGAAGAAAAGG
CTATTGAAAGAGAGGTCTTACTTGTTCCTGGAAATGGTTTCTTCATTGATGGCTCAGCTCCTACCTCCTT
CTTCAGAGCATCCTTCTCTCTGGCTACTCCAGCACAGATGGATACAGCCTTCCAGAGATTGGCCCAACTG
ATAAAAGAGTCTTTA


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG206782 representing NM_011834
Red=Cloning site Green=Tags(s)

MNYSRFLTATSLARKPSPIRTTADILSKAPKTLISLAPGSPNPSMFPFKSAAFTVENGSTIRFEDDLIKR
ALQYSPSYGIPELLSWLKQFQVKLHNPPTVNYPPNQGQMDLCITSGCQDGLCKAFEMLINPGDTILVNEP
LFPGTLYAMKPLGCNIINVPSDEHGIIPEGLKKILSQWKPEDSKDPTKKTPKFLYTVPNGNNPTGNSLTG
DRKKEIYELARKYDFLIIEDDPYYFLQFSKPWEPTFLSMDVDGRVIRADTFSKTVSSGLRVGFMTGPKTL
IQNIVLHTQVSSVHACTLSQLMILQLLHQWGEEGFLAHIDRTIDFYKNQRDSILAAADKWLRGLAEWHVP
KAGMFLWIKVKGISDTKQLIEEKAIEREVLLVPGNGFFIDGSAPTSFFRASFSLATPAQMDTAFQRLAQL
IKESL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_011834
ORF Size 1275 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_011834.2, NP_035964.1
RefSeq Size 1780 bp
RefSeq ORF 1278 bp
Locus ID 23923
UniProt ID Q9WVM8
Cytogenetics 8 30.85 cM
Gene Summary Transaminase with broad substrate specificity. Has transaminase activity towards aminoadipate, kynurenine, methionine and glutamate. Shows activity also towards tryptophan, aspartate and hydroxykynurenine. Accepts a variety of oxo-acids as amino-group acceptors, with a preference for 2-oxoglutarate, 2-oxocaproic acid, phenylpyruvate and alpha-oxo-gamma-methiol butyric acid. Can also use glyoxylate as amino-group acceptor (in vitro) (By similarity).[UniProtKB/Swiss-Prot Function]

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