Ctbp2 (BC013333) Mouse Tagged ORF Clone

CAT#: MG206675

Ctbp2 (tGFP-tagged) - Mouse C-terminal binding protein 2 (cDNA clone MGC:13751 IMAGE:4019572)

Product Images

Circular map for MG206675

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ctbp2
• Synonyms: AA407280; D7Ertd45e; Gtrgeo6; Ribeye
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG206675 representing BC013333
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACGGCCCCCTGCACCCCCGCCCCCTGGTGGCACTGCTGGATGGCAGAGACTGCACTGTGGAGATGC
  CCATCCTGAAGGACCTGGCCACTGTGGCCTTCTGTGATGCACAGTCCACTCAGGAAATCCATGAGAAGGT
  GTTGAATGAGGCTGTGGGTGCCATGATGTACCACACCATCACCCTCACCAGGGAAGACCTGGAGAAGTTC
  AAGGCCCTGAGAGTGATCGTGCGAATCGGTAGTGGCTACGACAACGTGGACATCAAGGCTGCTGGCGAGC
  TCGGGATCGCTGTGTGCAACATCCCATCTGCTGCAGTGGAGGAGACAGCAGATTCGACAGTCTGCCACAT
  TCTCAATCTGTATCGGCGGAACACATGGCTCTACCAGGCCCTCCGAGAAGGCACGCGAGTACAGAGTGTG
  GAACAGATCCGTGAGGTCGCCTCAGGAGCTGCTCGGATCCGAGGGGAAACACTGGGCCTCATTGGCTTTG
  GTCGCACAGGGCAGGCAGTTGCTGTTCGAGCCAAGGCCTTTGGATTCAGCGTCATATTTTATGACCCCTA
  CTTACAGGATGGGATAGAGCGATCTCTGGGCGTGCAAAGGGTCTACACCCTGCAGGACTTGCTATATCAG
  AGCGACTGCGTCTCCTTGCACTGCAACCTGAACGAGCACAACCATCACCTCATCAATGACTTCACTATCA
  AGCAGATGAGGCAAGGTGCATTCCTTGTGAATGCAGCCCGAGGTGGCCTGGTGGATGAGAAAGCCTTAGC
  TCAGGCCCTCAAGGAGGGCAGGATACGAGGAGCTGCCCTGGATGTGCACGAGTCTGAGCCCTTCAGCTTC
  GCCCAGGGCCCATTGAAGGATGCTCCAAATCTCATCTGCACACCACACACAGCCTGGTACAGTGAACAAG
  CATCACTAGAGATGAGGGAAGCAGCAGCCACTGAGATCCGCCGAGCAATCACAGGTCGCATCCCAGAAAG
  CTTACGAAACTGTGTCAACAAAGAATTCTTCGTGACTTCAGCTCCTTGGTCAGTCATTGACCAGCAAGCA
  ATTCACCCCGAGCTCAATGGTGCCACATACAGGTATCCACCCGGCATTGTAGGTGTGGCTCCAGGAGGGC
  TTCCTCCAGCGATGGAAGGGATCATCCCTGGGGGCATCCCCGTGACTCACAACCTCCCCACAGTGGCACA
  CCCCTCCCAAGCTCCTTCCCCCAACCAGCCCACAAAACACGGGGACAATCGAGAGCACCCCAATGAGCAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG206675 representing BC013333
  Red=Cloning site Green=Tags(s)
  
  MNGPLHPRPLVALLDGRDCTVEMPILKDLATVAFCDAQSTQEIHEKVLNEAVGAMMYHTITLTREDLEKF
  KALRVIVRIGSGYDNVDIKAAGELGIAVCNIPSAAVEETADSTVCHILNLYRRNTWLYQALREGTRVQSV
  EQIREVASGAARIRGETLGLIGFGRTGQAVAVRAKAFGFSVIFYDPYLQDGIERSLGVQRVYTLQDLLYQ
  SDCVSLHCNLNEHNHHLINDFTIKQMRQGAFLVNAARGGLVDEKALAQALKEGRIRGAALDVHESEPFSF
  AQGPLKDAPNLICTPHTAWYSEQASLEMREAAATEIRRAITGRIPESLRNCVNKEFFVTSAPWSVIDQQA
  IHPELNGATYRYPPGIVGVAPGGLPPAMEGIIPGGIPVTHNLPTVAHPSQAPSPNQPTKHGDNREHPNEQ
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC013333
• ORF Size: 1260 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC013333.1
• RefSeq Size: 1982 bp
• RefSeq ORF: 1262 bp
• Locus ID: 13017
• Cytogenetics: 7 76.32 cM
• Gene Summary: Corepressor targeting diverse transcription regulators. Isoform 2 probably acts as a scaffold for specialized synapses (By similarity). Functions in brown adipose tissue (BAT) differentiation.[UniProtKB/Swiss-Prot Function]