Pnpla3 (NM_054088) Mouse Tagged ORF Clone

CAT#: MG206522

Pnpla3 (tGFP-tagged) - Mouse patatin-like phospholipase domain containing 3 (Pnpla3)

Product Images

Circular map for MG206522

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Pnpla3
• Synonyms: Adpn
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG206522 representing NM_054088
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTATGACCCAGAGCGCCGCTGGAGCCTGTCGTTTGCAGGCTGCGGCTTCCTGGGCTTCTACCACGTCG
  GGGCTACGCTATGTCTGAGCGAGCGCGCCCCGCACCTCCTCCGCGATGCGCGCACTTTCTTTGGCTGCTC
  GGCCGGTGCACTGCACGCGGTCACCTTCGTGTGCAGTCTCCCTCTCGGCCGTATAATGGAGATCCTCATG
  GACCTCGTGCGGAAAGCCAGGAGCCGCAACATCGGCACCCTCCACCCGTTCTTCAACATTAACAAGTGCA
  TCAGAGACGGGCTCCAGGAGAGCCTCCCAGACAATGTCCACCAGGTCATTTCTGGCAAGGTTCACATCTC
  ACTCACCAGGGTGTCGGATGGGGAGAACGTGCTGGTGTCTGAGTTCCATTCCAAAGACGAAGTCGTGGAT
  GCCCTGGTGTGTTCCTGCTTCATTCCCCTCTTCTCTGGCCTAATCCCTCCTTCCTTCCGAGGCGAGCGGT
  ACGTGGACGGAGGAGTGAGCGACAACGTCCCTGTGCTGGATGCCAAAACCACCATCACGGTGTCACCTTT
  CTACGGTGAGCATGACATCTGCCCCAAAGTCAAGTCCACCAACTTCTTCCACGTGAATATCACCAACCTC
  AGCCTCCGCCTCTGCACTGGGAACCTCCAACTTCTGACCAGAGCGCTCTTCCCGTCTGATGTGAAGGTGA
  TGGGAGAGCTGTGCTATCAAGGGTACCTGGACGCCTTCCGGTTCCTGGAGGAGAATGGCATCTGTAACGG
  GCCACAGCGCAGCCTGAGTCTGTCCTTGGTGGCGCCAGAAGCCTGCTTGGAAAATGGCAAACTTGTGGGA
  GACAAGGTGCCAGTCAGCCTATGCTTTACAGATGAGAACATCTGGGAGACACTGTCCCCCGAGCTCAGCA
  CAGCTCTGAGTGAAGCGATTAAGGACAGGGAGGGCTACCTGAGCAAAGTCTGCAACCTCCTGCCCGTCAG
  GATCCTGTCCTACATCATGCTGCCCTGCAGTCTGCCCGTGGAGTCGGCTATCGCTGCAGTCCACAGGCTG
  GTGACATGGCTCCCTGATATCCAGGATGATATCCAGTGGCTACAATGGGCGACATCCCAGGTTTGTGCCC
  GAATGACGATGTGCCTGCTCCCCTCTACCAGATCCCGAGCATCCAAGGATGACCATCGAATGCTCAAGCA
  TGGCCATCACCCATCTCCCCACAAGCCTCAAGGCAACTCTGCTGGTTTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG206522 representing NM_054088
  Red=Cloning site Green=Tags(s)
  
  MYDPERRWSLSFAGCGFLGFYHVGATLCLSERAPHLLRDARTFFGCSAGALHAVTFVCSLPLGRIMEILM
  DLVRKARSRNIGTLHPFFNINKCIRDGLQESLPDNVHQVISGKVHISLTRVSDGENVLVSEFHSKDEVVD
  ALVCSCFIPLFSGLIPPSFRGERYVDGGVSDNVPVLDAKTTITVSPFYGEHDICPKVKSTNFFHVNITNL
  SLRLCTGNLQLLTRALFPSDVKVMGELCYQGYLDAFRFLEENGICNGPQRSLSLSLVAPEACLENGKLVG
  DKVPVSLCFTDENIWETLSPELSTALSEAIKDREGYLSKVCNLLPVRILSYIMLPCSLPVESAIAAVHRL
  VTWLPDIQDDIQWLQWATSQVCARMTMCLLPSTRSRASKDDHRMLKHGHHPSPHKPQGNSAGL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_054088
• ORF Size: 1239 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_054088.2
• RefSeq Size: 3007 bp
• RefSeq ORF: 1155 bp
• Locus ID: 116939
• UniProt ID: Q91WW7
• Cytogenetics: 15 E2
• Gene Summary: Specifically catalyzes coenzyme A (CoA)-dependent acylation of 1-acyl-sn-glycerol 3-phosphate (2-lysophosphatidic acid/LPA) to generate phosphatidic acid (PA), an important metabolic intermediate and precursor for both triglycerides and glycerophospholipids. Does not esterify other lysophospholipids. Acyl donors are long chain (at least C16) fatty acyl-CoAs: arachidonoyl-CoA, linoleoyl-CoA, oleoyl-CoA and at a lesser extent palmitoyl-CoA (PubMed:22560221). Additionally possesses low triacylglycerol lipase and CoA-independent acylglycerol transacylase activities and thus may play a role in acyl-chain remodeling of triglycerides (By similarity).[UniProtKB/Swiss-Prot Function]