Ikbkg (NM_010547) Mouse Tagged ORF Clone

CAT#: MG206493

Ikbkg (tGFP-tagged) - Mouse inhibitor of kappaB kinase gamma (Ikbkg), transcript variant 1

Product Images

Circular map for MG206493

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ikbkg
• Synonyms: 1110037D23Rik; AI848108; AI851264; AW124339; IKK[g]; NEMO
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG206493 representing NM_010547
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACAAGCACCCCTGGAAGAACCAGCTGAGTGAGATGGTGCAGCCCAGTGGTGGCCCAGCAGAGGACC
  AGGACATGCTGGGTGAAGAATCTTCTCTGGGGAAGCCTGCAATGCTACATCTGCCTTCAGAGCAGGGTAC
  TCCTGAGACCCTCCAGCGCTGCCTGGAAGAGAATCAAGAGCTCCGAGACGCTATCCGGCAGAGCAATCAG
  ATGCTGAGGGAACGCTGTGAGGAGCTGCTGCATTTCCAGGTCAGCCAGCGGGAGGAGAAGGAGTTCCTTA
  TGTGCAAATTCCAGGAAGCCCGGAAGCTGGTGGAGAGACTGAGCTTGGAGAAGCTTGATCTTCGGAGTCA
  GAGGGAACAGGCCTTAAAGGAGTTGGAGCAACTGAAGAAATGCCAACAGATGGCTGAGGACAAGGCCTCT
  GTGAAAGCTCAGGTGACATCATTGCTCGGAGAACTCCAGGAGAGCCAGAGCCGTTTGGAGGCTGCCACCA
  AGGATCGGCAAGCTTTAGAGGGAAGGATTCGAGCAGTTAGTGAGCAGGTCAGACAGCTGGAGAGTGAGCG
  GGAGGTGCTACAGCAGCAGCACAGCGTCCAGGTGGACCAGCTGCGTATGCAGAACCAGAGCGTGGAGGCT
  GCCTTGCGAATGGAGCGGCAGGCTGCTTCAGAGGAGAAGCGGAAGCTGGCTCAGTTGCAGGCAGCCTATC
  ACCAGCTCTTCCAAGACTACGACAGCCACATTAAGAGCAGCAAGGGCATGCAGCTGGAAGATCTGAGGCA
  ACAGCTCCAGCAAGCTGAGGAGGCCCTGGTAGCCAAACAGGAATTGATTGATAAGCTGAAAGAGGAGGCT
  GAGCAGCACAAGATTGTGATGGAGACTGTGCCAGTCTTGAAGGCCCAGGCGGATATCTACAAGGCTGACT
  TCCAAGCTGAGAGGCATGCCCGGGAGAAGCTGGTGGAGAAGAAGGAGTATTTGCAGGAGCAGCTGGAGCA
  GCTGCAGCGCGAGTTCAACAAGCTGAAAGTTGGCTGCCATGAGTCAGCCAGGATTGAGGATATGAGGAAG
  CGGCATGTAGAGACTCCCCAGCCTCCTTTACTCCCTGCTCCAGCTCACCACTCCTTTCATTTGGCCTTGT
  CCAACCAGCGGAGGAGCCCTCCTGAAGAACCTCCTGACTTCTGTTGTCCGAAGTGCCAGTATCAGGCTCC
  TGATATGGACACTCTACAGATACATGTCATGGAGTGCATAGAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG206493 representing NM_010547
  Red=Cloning site Green=Tags(s)
  
  MNKHPWKNQLSEMVQPSGGPAEDQDMLGEESSLGKPAMLHLPSEQGTPETLQRCLEENQELRDAIRQSNQ
  MLRERCEELLHFQVSQREEKEFLMCKFQEARKLVERLSLEKLDLRSQREQALKELEQLKKCQQMAEDKAS
  VKAQVTSLLGELQESQSRLEAATKDRQALEGRIRAVSEQVRQLESEREVLQQQHSVQVDQLRMQNQSVEA
  ALRMERQAASEEKRKLAQLQAAYHQLFQDYDSHIKSSKGMQLEDLRQQLQQAEEALVAKQELIDKLKEEA
  EQHKIVMETVPVLKAQADIYKADFQAERHAREKLVEKKEYLQEQLEQLQREFNKLKVGCHESARIEDMRK
  RHVETPQPPLLPAPAHHSFHLALSNQRRSPPEEPPDFCCPKCQYQAPDMDTLQIHVMECIE
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010547
• ORF Size: 6811 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010547.1
• RefSeq Size: 1748 bp
• RefSeq ORF: 1239 bp
• Locus ID: 16151
• UniProt ID: O88522
• Cytogenetics: X A7.3
• Gene Summary: Regulatory subunit of the IKK core complex which phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Its binding to scaffolding polyubiquitin seems to play a role in IKK activation by multiple signaling receptor pathways. Also considered to be a mediator for TAX activation of NF-kappa-B. Could be implicated in NF-kappa-B-mediated protection from cytokine toxicity. Involved in TLR3- and IFIH1-mediated antiviral innate response; this function requires 'Lys-27'-linked polyubiquitination.[UniProtKB/Swiss-Prot Function]