Acy1 (NM_025371) Mouse Tagged ORF Clone

CAT#: MG206415

Acy1 (GFP-tagged) - Mouse aminoacylase 1 (Acy1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Acy1
• Synonyms: 1110014J22Rik; Acy-1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG206415 representing NM_025371
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCACCAAGGATCCCGAGTCTGAGCACCCCTCCGTGACGCTCTTCCGCCAGTACCTGCGCATCTGCA
  CTGTGCAACCCAATCCAGACTATGGAGGGGCCATCACCTTCCTGGAGGAGAGAGCCCGCCAGCTGGGTCT
  GAGCTGTCAGAAAATAGAGGTGGTGCCTGGCTTTGTGATCACTGTGCTGACCTGGCCAGGCACCAACCCG
  TCACTCCCCTCCATCTTGCTAAACTCCCACACAGATGTGGTACCTGTCTTCAAGGAACATTGGCATCACG
  ACCCCTTTGAGGCCTTCAAGGATTCCGAGGGCTACATCTATGCTAGGGGCTCCCAGGACATGAAGAGTGT
  CAGCATCCAGTACCTGGAAGCTGTGAGGAGGCTGAAGAGCGAGGGCCACCGATTCCCTAGGACCATCCAC
  ATGACCTTTGTGCCTGATGAGGAGGTCGGAGGTCACAAGGGGATGGAGTTGTTTGTGAAGAGGCCTGAGT
  TCCAGGCTCTGCGGGCAGGCTTCGCTCTGGACGAGGGCCTGGCCAACCCCACGGATGCCTTTACTGTCTT
  TTATAGTGAGCGGAGTCCTTGGTGGGTACGGGTTACCAGCACTGGGAAGCCAGGCCATGCCTCACGCTTC
  ATTGAGGATACAGCTGCAGAGAAGTTGCATAAGGTTATAAGCTCCATCTTGGCATTCCGGGAGAAGGAAA
  GGCAGAGGCTGCAGGCGAACCCTCACCTGAAGGAAGGCGCTGTGACTTCGGTGAATCTGACCAAGCTTGA
  AGGTGGCGTGGCCTATAATGTGGTACCTGCCACTATGAGTGCCAGCTTTGACTTCCGAGTGGCACCAGAT
  GTAGACATGAAGGCCTTCGAGAAGCAGCTGCAGAGATGGTGCCAGGAAGCGGGAGAGGGGGTCACCTTTG
  AGTTTGCTCAGAAGTTTACAGAGCCTCGAATGACACCCACGGATGATTCAGATCCCTGGTGGGCAGCTTT
  CAGCGGAGCCTGCAAGGCAATGAACCTCACTCTGGAGCCAGAGATCTTTCCTGCTGCCACTGACAGCCGC
  TATATCCGAGCGGTGGGGATCCCAGCTCTGGGCTTCTCGCCCATGAACCGCACACCTGTCCTGCTACATG
  ACCATAATGAACGGCTGCATGAGGATATATTTCTCCGTGGGGTTGACATATATACAGGCCTCCTCTCTGC
  ACTTGCTAGTGTGCCCACCCTGCCTGGTGAAAGT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG206415 representing NM_025371
  Red=Cloning site Green=Tags(s)
  
  MTTKDPESEHPSVTLFRQYLRICTVQPNPDYGGAITFLEERARQLGLSCQKIEVVPGFVITVLTWPGTNP
  SLPSILLNSHTDVVPVFKEHWHHDPFEAFKDSEGYIYARGSQDMKSVSIQYLEAVRRLKSEGHRFPRTIH
  MTFVPDEEVGGHKGMELFVKRPEFQALRAGFALDEGLANPTDAFTVFYSERSPWWVRVTSTGKPGHASRF
  IEDTAAEKLHKVISSILAFREKERQRLQANPHLKEGAVTSVNLTKLEGGVAYNVVPATMSASFDFRVAPD
  VDMKAFEKQLQRWCQEAGEGVTFEFAQKFTEPRMTPTDDSDPWWAAFSGACKAMNLTLEPEIFPAATDSR
  YIRAVGIPALGFSPMNRTPVLLHDHNERLHEDIFLRGVDIYTGLLSALASVPTLPGES
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_025371
• ORF Size: 1224 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025371.1, NP_079647.1
• RefSeq Size: 1432 bp
• RefSeq ORF: 1227 bp
• Locus ID: 109652
• UniProt ID: Q99JW2
• Cytogenetics: 9 57.49 cM
• Gene Summary: Involved in the hydrolysis of N-acylated or N-acetylated amino acids (except L-aspartate).[UniProtKB/Swiss-Prot Function]