Kcnab1 (NM_010597) Mouse Tagged ORF Clone
CAT#: MG206299
- TrueORF®
Kcnab1 (tGFP-tagged) - Mouse potassium voltage-gated channel, shaker-related subfamily, beta member 1 (Kcnab1)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_010597" in other vectors (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Kcnab1 |
Synonyms | Akr8a8; Kvbeta1.1; mKv(beta)1 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG206299 representing NM_010597
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAAGTCTCCATAGCCTGCACAGAGCACAATTTGAAGAGTCGAAATGGTGAGGACCGACTTCTGAGCA AGCAGAGCTCCAACGCCCCCAATGTGGTGAACGCAGCCCGGGCCAAATTCCGCACTGTCGCTATCATCGC TCGCAGCCTGGGGACCTTCACCCCTCAGCATCACATTTCTCTCAAAGAGTCCACCGCAAAGCAGACTGGC ATGAAATATAGGAATCTTGGGAAATCAGGACTCAGAGTTTCATGCTTGGGTCTTGGAACATGGGTGACAT TTGGAGGTCAAATCTCAGATGAGGTTGCTGAACGGCTGATGACAATTGCCTACGAAAGTGGAGTTAATCT CTTCGACACAGCTGAGGTCTATGCTGCTGGGAAGGCTGAGGTGATTCTGGGAAGCATCATCAAGAAGAAA GGCTGGAGGAGGTCCAGCTTGGTCATCACAACCAAACTCTACTGGGGTGGAAAAGCTGAGACAGAAAGGG GACTGTCAAGAAAGCACATCATTGAAGGACTGAAAGGCTCCCTCCAGAGGCTGCAACTGGAATACGTGGA TGTGGTCTTTGCAAATCGCCCAGACAGCAACACTCCCATGGAAGAAATCGTTCGAGCCATGACGCACGTG ATCAACCAAGGCATGGCCATGTACTGGGGCACCTCGAGGTGGAGCGCGATGGAGATCATGGAAGCCTACT CTGTCGCACGGCAGTTCAACATGATCCCGCCTGTCTGTGAGCAAGCTGAGTACCATCTTTTCCAGAGAGA GAAGGTGGAGGTCCAGCTGCCGGAGCTCTACCATAAAATAGGAGTTGGTGCAATGACATGGTCTCCACTT GCTTGTGGAATTATTTCAGGAAAATATGGAAATGGGGTGCCAGAAAGTTCTAGAGCTTCACTGAAGTGCT ACCAGTGGTTGAAGGAAAGAATCGTAAGTGAAGAAGGGAGAAAACAGCAAAACAAGCTGAAAGACCTCTC TCCAATCGCTGAGCGCCTGGGGTGCACGCTACCTCAGCTGGCTGTGGCGTGGTGCCTGAGAAATGAGGGT GTGAGTTCTGTGCTCCTGGGATCATCCACTCCGGAACAACTCATTGAAAACCTTGGTGCCATTCAGGTCC TCCCTAAGATGACATCTCACGTGGTGAACGAGATTGATAACATACTGCGCAACAAGCCCTACAGCAAAAA GGACTATAGATCA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG206299 representing NM_010597
Red=Cloning site Green=Tags(s) MQVSIACTEHNLKSRNGEDRLLSKQSSNAPNVVNAARAKFRTVAIIARSLGTFTPQHHISLKESTAKQTG MKYRNLGKSGLRVSCLGLGTWVTFGGQISDEVAERLMTIAYESGVNLFDTAEVYAAGKAEVILGSIIKKK GWRRSSLVITTKLYWGGKAETERGLSRKHIIEGLKGSLQRLQLEYVDVVFANRPDSNTPMEEIVRAMTHV INQGMAMYWGTSRWSAMEIMEAYSVARQFNMIPPVCEQAEYHLFQREKVEVQLPELYHKIGVGAMTWSPL ACGIISGKYGNGVPESSRASLKCYQWLKERIVSEEGRKQQNKLKDLSPIAERLGCTLPQLAVAWCLRNEG VSSVLLGSSTPEQLIENLGAIQVLPKMTSHVVNEIDNILRNKPYSKKDYRS TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_010597 |
ORF Size | 1203 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_010597.5 |
RefSeq Size | 3276 bp |
RefSeq ORF | 1206 bp |
Locus ID | 16497 |
UniProt ID | P63143 |
Cytogenetics | 3 30.15 cM |
Gene Summary | Cytoplasmic potassium channel subunit that modulates the characteristics of the channel-forming alpha-subunits (PubMed:10454353). Modulates action potentials via its effect on the pore-forming alpha subunits (PubMed:10454353). Promotes expression of the pore-forming alpha subunits at the cell membrane, and thereby increases channel activity (PubMed:8824288). Mediates closure of delayed rectifier potassium channels by physically obstructing the pore via its N-terminal domain and increases the speed of channel closure for other family members (By similarity). Promotes the closure of KCNA1, KCNA2 and KCNA5 channels (By similarity). Accelerates KCNA4 channel closure (By similarity). Accelerates the closure of heteromeric channels formed by KCNA1 and KCNA4 (By similarity). Accelerates the closure of heteromeric channels formed by KCNA2, KCNA5 and KCNA6 (By similarity). Enhances KCNB1 and KCNB2 channel activity (PubMed:8824288). Binds NADPH; this is required for efficient down-regulation of potassium channel activity (By similarity). Has NADPH-dependent aldoketoreductase activity (By similarity). Oxidation of the bound NADPH strongly decreases N-type inactivation of potassium channel activity (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC206015 | Kcnab1 (untagged) - Mouse potassium voltage-gated channel, shaker-related subfamily, beta member 1 (Kcnab1), (10ug) |
USD 686.00 |
|
MR206299 | Kcnab1 (Myc-DDK-tagged) - Mouse potassium voltage-gated channel, shaker-related subfamily, beta member 1 (Kcnab1) |
USD 686.00 |
|
MR206299L3 | Lenti ORF clone of Kcnab1 (Myc-DDK-tagged) - Mouse potassium voltage-gated channel, shaker-related subfamily, beta member 1 (Kcnab1) |
USD 986.00 |
|
MR206299L4 | Lenti ORF clone of Kcnab1 (mGFP-tagged) - Mouse potassium voltage-gated channel, shaker-related subfamily, beta member 1 (Kcnab1) |
USD 986.00 |
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