Acat2 (NM_009338) Mouse Tagged ORF Clone

CAT#: MG206240

Acat2 (tGFP-tagged) - Mouse acetyl-Coenzyme A acetyltransferase 2 (Acat2)

Product Images

Circular map for MG206240

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Acat2
• Synonyms: AW742799; Tcp-1x; Tcp1-rs1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG206240 representing NM_009338
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATGCGGGCTCAGACCCCGTGGTCATCGTCTCAGCGGCGCGCACCGCTATAGGCTCCTTCAATGGTG
  CCCTGTCCACCGTGCCTGTCCACGAGATGGGGACAACTGTCATCAAAGAAGTCCTGCAGAGGGCCAAGGT
  GGCTCCAGAAGAGGTGTCCGAGGTCATATTTGGACATGTCTTGACGGCAGGTTGTGGGCAGAATCCTACT
  CGACAAGCCAGTGTGGGTGCAGGGATCCCCTATTCGGTTCCAGCATGGAGCTGCCAGATGATCTGTGGCT
  CGGGCCTAAAAGCCGTGTGTCTTGCAGCCCAGTCCATAGCCATGGGCGACTCCACCATTGTGGTAGCTGG
  AGGCATGGAGAATATGAGCAAGGCTCCTCACTTGACTCACCTGAGAACAGGAGTCAGGATGGGTGAGGTG
  CCACTGGCTGACAGCATACTCTGTGATGGTCTGACAGATGCCTTTCACAACTACCACATGGGCATCACAG
  CTGAAAACGTAGCCAAAAAATGGCAAGTGAGCAGAGAGGCCCAGGACAAGGTTGCAGTTCTGTCACAGAA
  CAGGGCAGAGCATGCTCAGAAAGCTGGCCACTTTGACAAGGAGATTGTGCCAGTGCTGGTGTCTTCCAGA
  AAAGGTCTTACTGAAGTGAAAATCGATGAATTTCCCCGTCATGGGAGTAACCTTGAAGCCATGGGCAAGC
  TGAAACCTTACTTTCTTACTGATGGGACAGGAACTGTCACCCCAGCGAACGCATCAGGAATGAACGATGG
  CGCTGCTGCTGTGGTCCTTATGAAGAAGACAGAAGCTGAGAGGCGGATGCTGAAACCTTTAGCAAGAATA
  GTCTCCTGGTCCCAAGCCGGTGTGGAGCCTTCTGTTATGGGAGTAGGACCAATTCCAGCCATAAAGCAAG
  CTGTTGCAAAGGCAGGCTGGTCCCTGGAGGATGTTGACCTGTTTGAAATCAATGAAGCCTTTGCAGCGGT
  GTCTGCGGCAATAGCTAAAGAACTTGGATTAAACCCCGAGAAGGTCAACATCGATGGAGGAGCCATTGCC
  TTGGGCCATCCTCTGGGAGCATCTGGCTGCAGGATTCTAGTGACCTTGCTGCACACCCTGGAGAGAGTGG
  GCGGGACCCGTGGTGTTGCAGCCCTGTGCATTGGGGGTGGGATGGGAGTCGCAATGTGTGTTCAGAGAGG
  G
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG206240 representing NM_009338
  Red=Cloning site Green=Tags(s)
  
  MNAGSDPVVIVSAARTAIGSFNGALSTVPVHEMGTTVIKEVLQRAKVAPEEVSEVIFGHVLTAGCGQNPT
  RQASVGAGIPYSVPAWSCQMICGSGLKAVCLAAQSIAMGDSTIVVAGGMENMSKAPHLTHLRTGVRMGEV
  PLADSILCDGLTDAFHNYHMGITAENVAKKWQVSREAQDKVAVLSQNRAEHAQKAGHFDKEIVPVLVSSR
  KGLTEVKIDEFPRHGSNLEAMGKLKPYFLTDGTGTVTPANASGMNDGAAAVVLMKKTEAERRMLKPLARI
  VSWSQAGVEPSVMGVGPIPAIKQAVAKAGWSLEDVDLFEINEAFAAVSAAIAKELGLNPEKVNIDGGAIA
  LGHPLGASGCRILVTLLHTLERVGGTRGVAALCIGGGMGVAMCVQRG
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009338
• ORF Size: 1191 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009338.1, NP_033364.1
• RefSeq Size: 2215 bp
• RefSeq ORF: 1194 bp
• Locus ID: 110460
• UniProt ID: Q8CAY6
• Cytogenetics: 17 8.73 cM
• Gene Summary: Involved in the biosynthetic pathway of cholesterol.[UniProtKB/Swiss-Prot Function]