Vps37a (BC039290) Mouse Tagged ORF Clone

CAT#: MG206233

Vps37a (tGFP-tagged) - Mouse vacuolar protein sorting 37A (yeast) (cDNA clone MGC:37724 IMAGE:5066508)

Product Images

Circular map for MG206233

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Vps37a
• Synonyms: 2210018P21Rik; 4930592A21Rik; AW261445; D8Ertd531e
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG206233 representing BC039290
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCTGGCTCTTTCCCCTGGCGAAGAGCGCGTCATCCTCCGCGGCTGGGTCCCCTGCCGGCCTCACCA
  GTCTCCAGCAGCAGAAGCAGCGGCTGATCGAGTCACTCCGGAACTCCCATTCCAGTATAGCTGAAATACA
  AAAAGATGTGGAATACAGATTGCCATTCACAGTAAACAACCTGACAATTAACATTAATATATTACTTCCT
  CCACAGTTTCCTCAGGAAAAGCCAGTGATTAGTGTTTATCCGCCAATCAGACATCATTTAATGGATAGTC
  AAGGATTATATGTTACCTCTCCATTAGTAAGCAATTTTACAATGCACTCAGATCTTGGAAAAATTATTCA
  AAGCCTTTTGGATGAGTTCTGGAAGAATCCTCCAGTTTTAGCTCCTACTTCAACAACATTTCCATACCTG
  TATAGTAATCCAGGTGGGATGCCTCCGTACCCCTCCCAGGGGTTTCCATTTCTTCCTCCATATCCTCCAC
  CAGAAGCTAACAGGAATATCACATCCTTGTCTGTAGCTGACACTGTTTCTTCTTCAACCACAAGTTACAC
  TGCAGCTAAGCCCGTGGCGCCTTCATTTGGCATCCTTTCAAGTCTGCCATTACCAGTTCCCACAACTGAA
  TCTTCAGCATCGGTTAACCAAAATGGTTTTGGTTATAAGATGCCGGATATTCCTGATGCATTTCCAGAAC
  TTTCAGAACTAAGTGTGTCACAGCTCACAGATATGAATGAACAGGAGGAGGTATTACTAGAACAGTTTCT
  GATGTTGCCTCAACTAAAACAAATTATTACTGACAAGGAAGACTTAGTAAAAAATATCGAGGAATTAGCA
  AGAAAAAATCTCCTTTTGGAACATAGCTTGGAAGGCAAAAGGCAAACCGTTTTAGATAAGTATGAATTAC
  TTCTACAAATGAAATCTACCTTTGAAAAGAAGATGCAAAGGCAACATGAACTCAGTGAGAGTTGTAGTGC
  AAGTGCTCTACAGGCAAGATTGAAAGTAGCTGCACACGAAGCTGAGGAAGAATCGGATAATATTGCTGAA
  GACTTCTTGGAGGGAAAAACTGAAATAGATGATTTTCTCAATAGCTTCAAGGAAAAGAGAACAATTTGCC
  ACTGTAGAAGAGCTAAAGAAGAGAAGCTTCACCAGGTGATAGCGATGCACAGCCAATTTCATGCTCCTCT
  C
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG206233 representing BC039290
  Red=Cloning site Green=Tags(s)
  
  MSWLFPLAKSASSSAAGSPAGLTSLQQQKQRLIESLRNSHSSIAEIQKDVEYRLPFTVNNLTININILLP
  PQFPQEKPVISVYPPIRHHLMDSQGLYVTSPLVSNFTMHSDLGKIIQSLLDEFWKNPPVLAPTSTTFPYL
  YSNPGGMPPYPSQGFPFLPPYPPPEANRNITSLSVADTVSSSTTSYTAAKPVAPSFGILSSLPLPVPTTE
  SSASVNQNGFGYKMPDIPDAFPELSELSVSQLTDMNEQEEVLLEQFLMLPQLKQIITDKEDLVKNIEELA
  RKNLLLEHSLEGKRQTVLDKYELLLQMKSTFEKKMQRQHELSESCSASALQARLKVAAHEAEEESDNIAE
  DFLEGKTEIDDFLNSFKEKRTICHCRRAKEEKLHQVIAMHSQFHAPL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC039290
• ORF Size: 1193 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC039290, AAH39290
• RefSeq Size: 4998 bp
• RefSeq ORF: 1193 bp
• Locus ID: 52348
• Cytogenetics: 8 23.89 cM
• Gene Summary: Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies. May be involved in cell growth and differentiation (By similarity).[UniProtKB/Swiss-Prot Function]