Acaa2 (NM_177470) Mouse Tagged ORF Clone

CAT#: MG206222

Acaa2 (tGFP-tagged) - Mouse acetyl-Coenzyme A acyltransferase 2 (mitochondrial 3-oxoacyl-Coenzyme A thiolase) (Acaa2)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Acaa2
• Synonyms: 0610011L04Rik; AI255831; AI265397; D18Ertd240e
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG206222 representing NM_177470
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCTGCTACGAGGTGTGTTCATCGTCGCTGCGAAGCGAACACCCTTTGGAGCTTACGGGGGCCTTC
  TCAAGGACTTCTCTGCCACCGATTTAACTGAATTTGCTGCCAGGGCTGCTCTGTCTGCTGGCAAAGTTCC
  ACCTGAAACCATCGATAGTGTCATCGTGGGCAATGTCATGCAGAGCTCTTCAGATGCGGCATACCTGGCG
  AGGCATGTGGGTTTGCGAGTGGGAGTCCCAACAGAGACTGGGGCACTTACCCTCAACAGGCTCTGTGGCT
  CTGGTTTCCAGTCCATCGTGAGCGGATGTCAGGAAATCTGTTCTAAAGATGCTGAGGTCGTCTTGTGTGG
  AGGAACAGAGAGCATGAGCCAGTCCCCCTACTGTGTCAGAAATGTGCGCTTCGGAACCAAATTTGGATTA
  GATCTCAAGCTGGAAGATACTTTGTGGGCAGGATTAACGGATCAACATGTTAAGCTGCCCATGGGAATGA
  CTGCAGAGAACCTTGCTGCAAAATACAACATAAGCAGAGAAGACTGTGACAGATACGCCTTGCAGTCTCA
  GCAGAGGTGGAAAGCTGCTAACGAGGCTGGCTACTTCAATGAGGAGATGGCACCCATTGAGGTGAAGACG
  AAGAAAGGCAAACAGACCATGCAAGTGGACGAGCACGCTCGACCCCAAACCACCCTGGAGCAACTGCAGA
  AGCTCCCGTCCGTGTTCAAGAAAGACGGGACAGTCACAGCAGGGAACGCCTCGGGGGTGTCTGACGGTGC
  TGGGGCCGTCATCATAGCCAGCGAAGATGCTGTCAAAAAACATAACTTCACGCCCCTGGCCAGAGTCGTG
  GGCTACTTCGTGTCCGGATGCGATCCTACTATCATGGGTATTGGTCCAGTCCCTGCTATCAATGGAGCAT
  TGAAGAAAGCTGGGCTGAGTCTTAAGGACATGGATTTGATAGACGTGAACGAAGCTTTTGCCCCTCAGTT
  CTTGTCTGTTCAGAAGGCCCTGGATCTTGACCCCAGCAAAACCAATGTGAGTGGAGGCGCCATTGCCCTG
  GGTCACCCGCTGGGAGGATCTGGCTCCAGAATCACCGCACACCTGGTTCATGAGTTAAGGCGTCGAGGTG
  GAAAGTACGCAGTGGGATCGGCTTGCATTGGAGGTGGCCAAGGCATCGCCCTGATCATCCAGAACACAGT
  C
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG206222 representing NM_177470
  Red=Cloning site Green=Tags(s)
  
  MALLRGVFIVAAKRTPFGAYGGLLKDFSATDLTEFAARAALSAGKVPPETIDSVIVGNVMQSSSDAAYLA
  RHVGLRVGVPTETGALTLNRLCGSGFQSIVSGCQEICSKDAEVVLCGGTESMSQSPYCVRNVRFGTKFGL
  DLKLEDTLWAGLTDQHVKLPMGMTAENLAAKYNISREDCDRYALQSQQRWKAANEAGYFNEEMAPIEVKT
  KKGKQTMQVDEHARPQTTLEQLQKLPSVFKKDGTVTAGNASGVSDGAGAVIIASEDAVKKHNFTPLARVV
  GYFVSGCDPTIMGIGPVPAINGALKKAGLSLKDMDLIDVNEAFAPQFLSVQKALDLDPSKTNVSGGAIAL
  GHPLGGSGSRITAHLVHELRRRGGKYAVGSACIGGGQGIALIIQNTV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_177470
• ORF Size: 1191 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177470.2, NP_803421.1
• RefSeq Size: 1502 bp
• RefSeq ORF: 1194 bp
• Locus ID: 52538
• UniProt ID: Q8BWT1
• Cytogenetics: 18 50.76 cM
• Gene Summary: In the production of energy from fats, this is one of the enzymes that catalyzes the last step of the mitochondrial beta-oxidation pathway, an aerobic process breaking down fatty acids into acetyl-CoA. Using free coenzyme A/CoA, catalyzes the thiolytic cleavage of medium- to long-chain unbranched 3-oxoacyl-CoAs into acetyl-CoA and a fatty acyl-CoA shortened by two carbon atoms. Also catalyzes the condensation of two acetyl-CoA molecules into acetoacetyl-CoA and could be involved in the production of ketone bodies. Also displays hydrolase activity on various fatty acyl-CoAs (By similarity). Thereby, could be responsible for the production of acetate in a side reaction to beta-oxidation (By similarity). Abolishes BNIP3-mediated apoptosis and mitochondrial damage (By similarity).[UniProtKB/Swiss-Prot Function]