S1pr1 (NM_007901) Mouse Tagged ORF Clone

CAT#: MG205968

S1pr1 (tGFP-tagged) - Mouse endothelial differentiation sphingolipid G-protein-coupled receptor 1 (Edg1)

Product Images

Circular map for MG205968

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: S1pr1
• Synonyms: AI849002; Edg1; Lpb1; S; S1; S1p; S1p1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG205968 representing NM_007901
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGTCCACTAGCATCCCGGAGGTTAAAGCTCTCCGCAGCTCAGTCTCTGACTATGGGAACTATGATA
  TCATAGTCCGGCATTACAACTACACAGGCAAGTTGAACATCGGGGCGGAGAAGGACCATGGCATTAAACT
  GACTTCAGTGGTGTTCATTCTCATCTGCTGCTTCATCATCCTAGAGAATATATTTGTCTTGCTAACTATT
  TGGAAAACCAAGAAGTTCCACCGGCCCATGTACTATTTCATAGGCAACCTAGCCCTCTCGGACCTATTAG
  CAGGCGTGGCTTACACAGCTAACCTGCTGTTGTCTGGGGCCACCACTTACAAGCTCACACCTGCCCAGTG
  GTTTCTGCGGGAAGGGAGTATGTTTGTGGCTCTCTCTGCATCAGTCTTCAGCCTCCTTGCCATCGCCATT
  GAGCGCTACATCACCATGCTGAAGATGAAACTACACAACGGGAGCAACAGCTCGCGCTCCTTTCTGCTGA
  TCAGCGCCTGCTGGGTCATCTCCCTCATCCTGGGGGGCCTGCCCATCATGGGCTGGAACTGCATCAGCTC
  GCTGTCTAGCTGCTCCACCGTGCTCCCGCTCTACCACAAGCACTATATTCTCTTCTGCACCACCGTCTTC
  ACTCTGCTCCTGCTTTCCATCGTCATCCTCTACTGCAGGATCTACTCCTTGGTCAGGACTCGAAGCCGCC
  GCCTGACCTTCCGCAAGAACATCTCCAAGGCCAGTCGCAGTTCTGAGAAGTCTCTGGCCTTGCTGAAGAC
  GGTGATCATTGTCCTGAGTGTCTTCATTGCCTGCTGGGCCCCTCTCTTCATCCTACTACTGTTAGATGTG
  GGCTGCAAGGCGAAGACCTGTGACATCCTGTACAAAGCAGAGTACTTCCTGGTTCTGGCTGTGCTGAACT
  CAGGTACCAACCCCATCATCTACACTCTGACCAACAAGGAGATGCGCCGGGCCTTCATCCGGATCGTATC
  TTGTTGCAAATGCCCCAACGGAGACTCTGCTGGCAAATTCAAGAGGCCCATCATCCCAGGCATGGAATTT
  AGCCGCAGCAAATCAGACAACTCCTCTCACCCCCAGAAGGACGATGGGGACAACCCAGAGACCATTATGT
  CGTCTGGAAACGTCAATTCTTCTTCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG205968 representing NM_007901
  Red=Cloning site Green=Tags(s)
  
  MVSTSIPEVKALRSSVSDYGNYDIIVRHYNYTGKLNIGAEKDHGIKLTSVVFILICCFIILENIFVLLTI
  WKTKKFHRPMYYFIGNLALSDLLAGVAYTANLLLSGATTYKLTPAQWFLREGSMFVALSASVFSLLAIAI
  ERYITMLKMKLHNGSNSSRSFLLISACWVISLILGGLPIMGWNCISSLSSCSTVLPLYHKHYILFCTTVF
  TLLLLSIVILYCRIYSLVRTRSRRLTFRKNISKASRSSEKSLALLKTVIIVLSVFIACWAPLFILLLLDV
  GCKAKTCDILYKAEYFLVLAVLNSGTNPIIYTLTNKEMRRAFIRIVSCCKCPNGDSAGKFKRPIIPGMEF
  SRSKSDNSSHPQKDDGDNPETIMSSGNVNSSS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007901
• ORF Size: 1146 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007901.5, NP_031927.2
• RefSeq Size: 3016 bp
• RefSeq ORF: 1149 bp
• Locus ID: 13609
• UniProt ID: O08530
• Cytogenetics: 3 G1
• Gene Summary: This gene encodes a G-protein-coupled receptor bound by the lysophospholipid, sphingosine 1-phosphate. The gene product functions in endothelial cells and is involved in vascular and heart development. This receptor is highly expressed in T and B lymphocytes, and it plays a role in T cell and B cell export from peripheral lymphoid organs. This protein is bound and downregulated by FTY720, an exogenous immunosuppressant drug studied in mouse disease models for multiple sclerosis in humans. [provided by RefSeq, Jan 2010]