Actc1 (BC062138) Mouse Tagged ORF Clone

CAT#: MG205866

Actc1 (tGFP-tagged) - Mouse actin, alpha, cardiac (cDNA clone MGC:69991 IMAGE:6516862)

Product Images

Circular map for MG205866

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Actc1
• Synonyms: Actc-1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG205866 representing BC062138
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGTGACGATGAGGAGACCACCGCTTTGGTGTGTGACAATGGCTCCGGGCTGGTGAAGGCCGGCTTTG
  CGGGTGATGACGCCCCCAGAGCTGTCTTCCCATCCATCGTGGGTCGCCCACGCCACCAGGGTGTCATGGT
  GGGTATGGGACAGAAGGACTCCTATGTAGGTGATGAAGCCCAGAGCAAGCGAGGTATCCTGACTCTGAAG
  TACCCCATTGAACATGGCATTATCACCAACTGGGATGACATGGAGAAGATCTGGCACCATACATTCTACA
  ATGAGCTCCGTGTGGCCCCAGAGGAACACCCAACCCTGCTCACTGAAGCCCCGCTGAACCCCAAGGCCAA
  CCGTGAGAAGATGACCCAGATCATGTTTGAAACCTTCAATGTGCCTGCCATGTATGTCGCCATCCAGGCA
  GTGCTGTCTCTGTATGCTTCTGGAAGAACCACAGGCATTGTTCTGGATTCTGGCGATGGTGTAACTCATA
  ATGTTCCCATCTATGAGGGTTATGCCTTACCCCATGCCATCATGCGCCTGGATCTAGCTGGTCGGGACCT
  CACTGACTACCTCATGAAGATCCTCACTGAGCGCGGGTACTCCTTTGTCACCACTGCTGAACGTGAAATT
  GTCCGTGACATAAAAGAAAAACTGTGTTACGTCGCCCTGGATTTTGAGAACGAGATGGCCACAGCTGCAT
  CTTCCTCCTCTCTGGAGAAGAGCTATGAACTTCCTGACGGCCAGGTCATCACCATTGGCAACGAGCGCTT
  CCGCTGTCCAGAGACTCTCTTCCAGCCCTCTTTCATTGGTATGGAATCTGCTGGCATTCATGAAACTACT
  TACAACAGCATCATGAAGTGTGACATCGATATCCGCAAAGACCTGTATGCCAACAATGTCCTATCTGGAG
  GCACCACCATGTACCCTGGTATTGCCGATCGTATGCAAAAGGAAATCACTGCACTGGCTCCCAGCACCAT
  GAAGATCAAGATTATTGCTCCCCCTGAGCGTAAATACTCTGTCTGGATTGGGGGCTCCATCCTGGCCTCT
  CTGTCCACCTTCCAGCAAATGTGGATCAGCAAGCAAGAGTATGATGAGGCAGGCCCATCCATTGTCCACC
  GCAAGTGCTTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG205866 representing BC062138
  Red=Cloning site Green=Tags(s)
  
  MCDDEETTALVCDNGSGLVKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLK
  YPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQA
  VLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREI
  VRDIKEKLCYVALDFENEMATAASSSSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETT
  YNSIMKCDIDIRKDLYANNVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILAS
  LSTFQQMWISKQEYDEAGPSIVHRKCF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC062138
• ORF Size: 1131 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC062138.1
• RefSeq Size: 1454 bp
• RefSeq ORF: 1133 bp
• Locus ID: 11464
• Cytogenetics: 2 57.55 cM
• Gene Summary: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.[UniProtKB/Swiss-Prot Function]