Agtr1a (NM_177322) Mouse Tagged ORF Clone

CAT#: MG205480

Agtr1a (tGFP-tagged) - Mouse angiotensin II receptor, type 1a (Agtr1a)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Agtr1a
• Synonyms: 1810074K20Rik; AG2S; Agtr-1a; Agtr1; AI551199; Angtr-1a; AT1; AT1a; AT2R1; AT2R1A
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG205480 representing NM_177322
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCTTAACTCTTCTACTGAAGATGGCATCAAAAGAATTCAAGATGACTGCCCCAGGGCTGGCAGGC
  ACAGTTACATATTTGTCATGATCCCTACTCTCTACAGCATCATCTTTGTGGTGGGAATATTTGGAAACAG
  CTTGGTGGTGATCGTCATCTACTTTTACATGAAGCTGAAGACTGTGGCCAGTGTCTTTCTTCTAAATCTC
  GCCCTGGCTGACTTATGCTTTTTGCTGACTTTGCCCCTGTGGGCTGTCTATACCGCTATGGAATACCGCT
  GGCCCTTCGGCAATCACCTATGTAAGATCGCTTCGGCCAGCGTCAGTTTCAACCTCTACGCCAGCGTGTT
  CCTGCTCACGTGTCTCAGCATCGATCGCTACCTGGCCATTGTCCACCCGATGAAGTCTCGCCTCCGCCGC
  ACGATGCTGGTGGCCAAAGTCACCTGCATCATCATCTGGCTGATGGCTGGCTTGGCCAGTTTGCCAGCCG
  TCATCCACCGAAATGTGTATTTCATTGAGAACACCAATATCACTGTTTGCGCTTTTCATTACGAGTCCCG
  GAATTCAACGCTCCCCATAGGACTGGGCCTAACCAAGAACATCCTGGGCTTCCTGTTCCCTTTCCTAATC
  ATTCTTACCAGCTATACTCTTATATGGAAAGCTCTAAAGAAGGCTTATGAAATTCAGAAGAACAAGCCAA
  GAAATGATGACATCTTTAGGATAATTATGGCGATTGTGCTTTTCTTCTTCTTCTCCTGGGTCCCCCACCA
  AATATTCACATTCCTGGATGTGCTGATTCAGCTGGGCGTCATCCATGACTGTAAAATTGCCGACATCGTG
  GACACTGCCATGCCCATAACCATCTGCATAGCGTATTTTAACAACTGCCTGAACCCTCTGTTTTACGGCT
  TTCTGGGGAAAAAATTTAAAAAGTATTTCCTCCAGCTCCTGAAATATATTCCCCCAAAGGCCAAGTCGCA
  CTCAAGCCTGTCTACGAAAATGAGCACGCTCTCCTACCGCCCCTCAGATAACATGAGCTCAGCCGCCAAA
  AAGCCTGCGTCTTGTTCTGAGGTGGAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG205480 representing NM_177322
  Red=Cloning site Green=Tags(s)
  
  MALNSSTEDGIKRIQDDCPRAGRHSYIFVMIPTLYSIIFVVGIFGNSLVVIVIYFYMKLKTVASVFLLNL
  ALADLCFLLTLPLWAVYTAMEYRWPFGNHLCKIASASVSFNLYASVFLLTCLSIDRYLAIVHPMKSRLRR
  TMLVAKVTCIIIWLMAGLASLPAVIHRNVYFIENTNITVCAFHYESRNSTLPIGLGLTKNILGFLFPFLI
  ILTSYTLIWKALKKAYEIQKNKPRNDDIFRIIMAIVLFFFFSWVPHQIFTFLDVLIQLGVIHDCKIADIV
  DTAMPITICIAYFNNCLNPLFYGFLGKKFKKYFLQLLKYIPPKAKSHSSLSTKMSTLSYRPSDNMSSAAK
  KPASCSEVE
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_177322
• ORF Size: 1077 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177322.3, NP_796296.1
• RefSeq Size: 2284 bp
• RefSeq ORF: 1080 bp
• Locus ID: 11607
• UniProt ID: P29754
• Cytogenetics: 13 13.19 cM
• Gene Summary: Receptor for angiotensin II. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.[UniProtKB/Swiss-Prot Function]