Arg2 (NM_009705) Mouse Tagged ORF Clone

CAT#: MG205396

Arg2 (tGFP-tagged) - Mouse arginase type II (Arg2)

Product Images

Circular map for MG205396

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Arg2
• Synonyms: AII; AU022422
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG205396 representing NM_009705
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCCTGAGGAGCAGCGCCTCCCGTCTCCTCCACGGGCAAATTCCTTGCGTCCTGACGAGATCCGTCC
  ACTCTGTAGCTATAGTCGGAGCCCCTTTCTCTCGGGGACAGAAGAAGCTAGGAGTGGAATATGGTCCAGC
  TGCCATTCGAGAAGCTGGCTTGCTGAAGAGGCTCTCCAGGTTGGGATGCCACCTAAAAGACTTTGGAGAC
  TTGAGTTTTACTAATGTCCCACAAGATAATCCCTACAATAATCTGGTTGTGTATCCTCGTTCAGTGGGCC
  TTGCCAACCAGGAACTGGCTGAAGTGGTTAGTAGAGCTGTGTCAGGTGGCTACAGCTGTGTCACCATGGG
  AGGAGACCACAGCCTGGCAATAGGTACCATTATCGGTCACGCCCGGCACCGCCCAGATCTCTGTGTCATC
  TGGGTTGATGCTCATGCGGACATTAATACACCTCTCACCACTGTATCTGGAAATATACATGGACAGCCAC
  TTTCCTTTCTCATCAAAGAACTACAAGACAAGGTACCACAACTGCCAGGATTTTCCTGGATCAAACCTTG
  CCTCTCTCCCCCAAATATTGTGTACATTGGCCTGAGAGATGTGGAGCCTCCTGAACATTTTATTTTAAAG
  AATTATGACATCCAGTATTTTTCCATGAGAGAGATTGATCGACTTGGGATCCAGAAGGTGATGGAACAGA
  CATTTGATCGGCTGATTGGCAAAAGGCAGAGGCCAATCCACCTGAGTTTTGATATTGATGCATTTGACCC
  TAAATTGGCTCCAGCCACAGGAACCCCTGTTGTAGGGGGATTAACCTACAGAGAAGGAGTGTATATTACT
  GAAGAAATACATAATACAGGGTTGCTGTCAGCTCTGGATCTTGTTGAAGTCAATCCTCATTTGGCCACTT
  CTGAGGAAGAGGCCAAGGCAACAGCCAGACTAGCAGTGGATGTGATTGCTTCAAGTTTTGGTCAGACAAG
  AGAAGGAGGACACATTGTCTATGACCACCTTCCTACTCCTAGTTCACCACACGAATCAGAAAATGAAGAA
  TGTGTGAGAATT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG205396 representing NM_009705
  Red=Cloning site Green=Tags(s)
  
  MFLRSSASRLLHGQIPCVLTRSVHSVAIVGAPFSRGQKKLGVEYGPAAIREAGLLKRLSRLGCHLKDFGD
  LSFTNVPQDNPYNNLVVYPRSVGLANQELAEVVSRAVSGGYSCVTMGGDHSLAIGTIIGHARHRPDLCVI
  WVDAHADINTPLTTVSGNIHGQPLSFLIKELQDKVPQLPGFSWIKPCLSPPNIVYIGLRDVEPPEHFILK
  NYDIQYFSMREIDRLGIQKVMEQTFDRLIGKRQRPIHLSFDIDAFDPKLAPATGTPVVGGLTYREGVYIT
  EEIHNTGLLSALDLVEVNPHLATSEEEAKATARLAVDVIASSFGQTREGGHIVYDHLPTPSSPHESENEE
  CVRI
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009705
• ORF Size: 1062 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009705.1, NP_033835.1
• RefSeq Size: 1400 bp
• RefSeq ORF: 1065 bp
• Locus ID: 11847
• UniProt ID: O08691
• Cytogenetics: 12 C3
• Gene Summary: May play a role in the regulation of extra-urea cycle arginine metabolism and also in down-regulation of nitric oxide synthesis. Extrahepatic arginase functions to regulate L-arginine bioavailability to nitric oxid synthase (NOS). Arginine metabolism is a critical regulator of innate and adaptive immune responses. Seems to be involved in negative regulation of the survival capacity of activated CD4(+) and CD8(+) T cells (PubMed:27745970, PubMed:25009204). May suppress inflammation-related signaling in asthmatic airway epithelium (PubMed:27214549). May contribute to the immune evasion of H.pylori by restricting M1 macrophage activation and polyamine metabolism (PubMed:27074721). May play a role in promoting prenatal immune suppression (By similarity). Regulates RPS6KB1 signaling, which promotes endothelial cell senescence and inflammation and implicates NOS3/eNOS dysfunction (PubMed:22928666). Can inhibit endothelial autophagy independently of its enzymatic activity implicating mTORC2 signaling (PubMed:25484082). Involved in vascular smooth muscle cell senescence and apoptosis independently of its enzymatic activity (By similarity).[UniProtKB/Swiss-Prot Function]