Anxa1 (NM_010730) Mouse Tagged ORF Clone

CAT#: MG205210

Anxa1 (tGFP-tagged) - Mouse annexin A1 (Anxa1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Anxa1
• Synonyms: Anx-1; Anx-A1; C430014K04Rik; Lpc-1; Lpc1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG205210 representing NM_010730
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAATGGTATCAGAATTCCTCAAGCAAGCCCGTTTTCTTGAAAATCAAGAACAGGAATATGTTCAAG
  CTGTAAAATCATACAAAGGTGGTCCTGGGTCAGCAGTGAGCCCCTACCCTTCCTTCAATGTATCCTCGGA
  TGTTGCTGCCTTGCACAAAGCTATCATGGTTAAAGGTGTGGATGAAGCAACCATCATTGACATTCTTACC
  AAGAGGACCAATGCTCAGCGCCAGCAGATCAAGGCCGCGTACTTACAGGAGAATGGAAAGCCCTTGGATG
  AAGTCTTGAGAAAAGCCCTTACAGGCCACCTGGAGGAGGTTGTTTTGGCTATGCTAAAAACTCCAGCTCA
  GTTTGATGCAGATGAACTCCGTGGTGCCATGAAGGGACTTGGAACAGATGAAGACACTCTCATTGAGATT
  TTGACAACAAGATCTAACGAACAAATCAGAGAGATTAATAGAGTCTACAGAGAAGAACTGAAAAGAGATC
  TGGCCAAAGACATCACTTCAGATACATCTGGAGACTTTCGGAAAGCCTTGCTTGCTCTTGCCAAGGGTGA
  CCGTTGTCAGGACTTGAGTGTGAATCAAGATTTGGCTGATACGGATGCCAGGGCTTTGTATGAAGCTGGA
  GAAAGGAGAAAGGGGACAGACGTGAACGTGTTCACTACAATTCTGACCAGTAGGAGCTTTCCTCATCTTC
  GCAGAGTGTTTCAGAATTACGGAAAGTACAGTCAACATGACATGAACAAAGCTCTGGATCTGGAACTGAA
  GGGTGACATTGAGAAGTGCCTCACAACCATCGTGAAGTGTGCCACCAGCACTCCAGCTTTCTTTGCCGAG
  AAGCTGTACGAAGCCATGAAGGGTGCCGGAACTCGCCATAAGGCATTGATCAGGATTATGGTCTCCCGTT
  CGGAAATTGACATGAATGAAATCAAAGTATTTTACCAGAAGAAGTATGGAATCTCTCTTTGCCAAGCCAT
  CCTGGATGAAACCAAAGGAGACTATGAAAAAATCCTGGTGGCTCTGTGTGGTGGAAAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG205210 representing NM_010730
  Red=Cloning site Green=Tags(s)
  
  MAMVSEFLKQARFLENQEQEYVQAVKSYKGGPGSAVSPYPSFNVSSDVAALHKAIMVKGVDEATIIDILT
  KRTNAQRQQIKAAYLQENGKPLDEVLRKALTGHLEEVVLAMLKTPAQFDADELRGAMKGLGTDEDTLIEI
  LTTRSNEQIREINRVYREELKRDLAKDITSDTSGDFRKALLALAKGDRCQDLSVNQDLADTDARALYEAG
  ERRKGTDVNVFTTILTSRSFPHLRRVFQNYGKYSQHDMNKALDLELKGDIEKCLTTIVKCATSTPAFFAE
  KLYEAMKGAGTRHKALIRIMVSRSEIDMNEIKVFYQKKYGISLCQAILDETKGDYEKILVALCGGN
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010730
• ORF Size: 1038 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010730.2, NP_034860.2
• RefSeq Size: 1395 bp
• RefSeq ORF: 1041 bp
• Locus ID: 16952
• UniProt ID: P10107
• Cytogenetics: 19 13.83 cM
• Gene Summary: Plays important roles in the innate immune response as effector of glucocorticoid-mediated responses and regulator of the inflammatory process. Has anti-inflammatory activity (PubMed:12475898). Plays a role in glucocorticoid-mediated down-regulation of the early phase of the inflammatory response (PubMed:12475898). Promotes resolution of inflammation and wound healing (PubMed:25664854). Functions at least in part by activating the formyl peptide receptors and downstream signaling cascades. Promotes chemotaxis of granulocytes and monocytes via activation of the formyl peptide receptors (By similarity). Contributes to the adaptive immune response by enhancing signaling cascades that are triggered by T-cell activation, regulates differentiation and proliferation of activated T-cells (PubMed:17948261). Promotes the differentiation of T-cells into Th1 cells and negatively regulates differentiation into Th2 cells (PubMed:17948261). Has no effect on unstimulated T-cells. Promotes rearrangement of the actin cytoskeleton, cell polarization and cell migration. Negatively regulates hormone exocytosis via activation of the formyl peptide receptors and reorganization of the actin cytoskeleton (By similarity). Has high affinity for Ca(2+) and can bind up to eight Ca(2+) ions (By similarity). Displays Ca(2+)-dependent binding to phospholipid membranes (By similarity). Plays a role in the formation of phagocytic cups and phagosomes (PubMed:21245195). Plays a role in phagocytosis by mediating the Ca(2+)-dependent interaction between phagosomes and the actin cytoskeleton (PubMed:21245195).[UniProtKB/Swiss-Prot Function]