Armc12 (NM_026290) Mouse Tagged ORF Clone

CAT#: MG205101

Armc12 (tGFP-tagged) - Mouse RIKEN cDNA 4930511I11 gene (4930511I11Rik)

Product Images

Circular map for MG205101

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Armc12
• Synonyms: 4930511I11Rik; AV041658
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG205101 representing NM_026290
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCAAGACCATTCCCCGGTTCCTGGAGCAACTGGACCTCATCAAAAGCTTCGTGGGCCTGGCTACAG
  GCGCCGGGGCCTTATACCTGCTGTACAAGGCCGTCAGGACTGGCTTAAAATGTCATCCGCCACTCTGCTC
  CAACTCGCCGATCTGCATTGCCCGCCTGGCCATCGAGAGAGAGCGCCACGGGCGGGACTCCGGTGAGATT
  CGAAGACTTCTCAACTCTCTAGACTGCAAACAGGATGAGTATACCAGGAGCATGATCCTTCATAACATCA
  CCCGCTGCGTGTACTTGCTGGAGGCTGAGGCCTCTTCTTGTACTATGGATGACATCGACTTGGTGGCTGA
  CATGCTAGATGAGAAGGACAACAGTGTAAAAATCCAAGCTCTGAATGCACTTAAAGCTTTCTCTGGCATC
  AGGAAATTCAGGCTCAAAATCCAGGAGCATTGCATCAAGGTACTGGAACTGATTTCCACCATCTGGGACT
  TGGAATTGCACGTAGCTGGTCTCCGATTGCTCAACAACCTCCCGCTACCTGACTATGTGCACCCACAGCT
  GCGGCGGGTGATGCCTGCCCTGATGGAGATCATACAGTCAGACTGCATCCTGGCACAGGTACAAGCTGTC
  CGCCTCCTGAGTTACCTGGCACAGAAGAACGACCTTCTTTATGACATTCTCAACTGTCAGGTGCGTCCCA
  ACTTCCTGAACCTCTTCCAGTCTTCACAGCCGGGCAGTCTGCTGTTCGAGGTGCTGGTGTTTGCGGAACA
  CTTGAGTGAAGGTCGGAACGCCACCCACTACCGAGCGGTCAAATGGCATTACAACGAGCAATCCCTGCAC
  GAAGCCCTCTTTGGGGATGAGTCCAGACTGGCAGACCGACTGCTCTCCCTGGTCATCCACCCTGAGGAGG
  AAGTTCAGATCCAGGCCTGCAAAGTCATAGTGAGCTTGCAGTGTCCCCAGGACCTGGGATCCCGACCCTC
  TTCCTGCCGACCTAGCCATTCCTGCTTTAAAACCGGGAAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG205101 representing NM_026290
  Red=Cloning site Green=Tags(s)
  
  MGKTIPRFLEQLDLIKSFVGLATGAGALYLLYKAVRTGLKCHPPLCSNSPICIARLAIERERHGRDSGEI
  RRLLNSLDCKQDEYTRSMILHNITRCVYLLEAEASSCTMDDIDLVADMLDEKDNSVKIQALNALKAFSGI
  RKFRLKIQEHCIKVLELISTIWDLELHVAGLRLLNNLPLPDYVHPQLRRVMPALMEIIQSDCILAQVQAV
  RLLSYLAQKNDLLYDILNCQVRPNFLNLFQSSQPGSLLFEVLVFAEHLSEGRNATHYRAVKWHYNEQSLH
  EALFGDESRLADRLLSLVIHPEEEVQIQACKVIVSLQCPQDLGSRPSSCRPSHSCFKTGK
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_026290
• ORF Size: 1020 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_026290.3, NP_080566.2
• RefSeq Size: 1103 bp
• RefSeq ORF: 1023 bp
• Locus ID: 67645
• UniProt ID: Q80X86
• Cytogenetics: 17 A3.3