Pex16 (NM_145122) Mouse Tagged ORF Clone

CAT#: MG204996

Pex16 (tGFP-tagged) - Mouse peroxisome biogenesis factor 16 (Pex16)

Product Images

Circular map for MG204996

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Pex16
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG204996 representing NM_145122
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAAGCTACGGCTCCTGAGCCTCCGCTACCAGGAGTATGTGACTCGTCATCCAGCCGCCACGGCCC
  AGTTGGAGACGGCTGTGCGGGGCCTCAGTTACCTGCTGGCAGGTCGCTTCTCCGATTCACACGAGCTGTC
  TGAACTGGTGTACTCTGCCTCGAACCTGCTGGTGCTGCTCAATGACGGGATCCTGAGGAAGGAGCTTCGA
  AAAAAGTTGCCTGTGTCGCTGTCCCAGCAGAAGTTGCTGACATGGCTGAGTGTACTGGAGTGTGTGGAGG
  TGTTCATGGAGATGGGGGCTGCCAAGGTGTGGGGCGAAGTAGGCCGTTGGCTCGTCATTGCTCTCATCCA
  GTTGGCCAAGGCCGTCCTGCGCATGCTCCTGCTGATCTGGTTCAAGGCTGGCATTCAGACGTCACCCCCC
  ATTGTTCCACTGGACAGGGAAACTCAGGCACAGCCCTTGGATGGTGACCACAATCCGGGCAGCCAGGAGC
  CATCATATGTGGGGAAACGGTCACACAGAGTGGTGCGAACCCTCCAGAACAGCCCATCCCTGCACTCACG
  GTACTGGGGAGCCCCTCAGCAGCGGGAAATTCGGCAGAAACAGCAGCAGGAGGAACTGAGCACACCCCCA
  ACACCTCTGGGGTTGCAGGAGACCATTGCAGAGTCTTTGTACATCGCCCGGCCCCTGCTGCACTTGCTCA
  GCCTGGGCTTATGGGGTCAGCGATCGTGGACACCCTGGCTACTGTCTGGTGTGGTAGATATGACTAGCCT
  GAGTCTCCTGAGTGACAGGAAGAACTTGACCCAGAGGGAGCGGCTAGAACTGAGGCGTCGCACAATCCTG
  CTGCTATACTACCTGCTACGCTCACCGTTCTATGACCGCTTCTCTGAAGCCAAGATCCTCTTCCTGCTCC
  AGCTGCTTACAGACCACATCCCTGGCGTGGGTCTGGTGGCAAGGCCGCTCATGGACTACTTGCCCTCCTG
  GCAGAAAATCTATTTCTACAGTTGGGGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG204996 representing NM_145122
  Red=Cloning site Green=Tags(s)
  
  MEKLRLLSLRYQEYVTRHPAATAQLETAVRGLSYLLAGRFSDSHELSELVYSASNLLVLLNDGILRKELR
  KKLPVSLSQQKLLTWLSVLECVEVFMEMGAAKVWGEVGRWLVIALIQLAKAVLRMLLLIWFKAGIQTSPP
  IVPLDRETQAQPLDGDHNPGSQEPSYVGKRSHRVVRTLQNSPSLHSRYWGAPQQREIRQKQQQEELSTPP
  TPLGLQETIAESLYIARPLLHLLSLGLWGQRSWTPWLLSGVVDMTSLSLLSDRKNLTQRERLELRRRTIL
  LLYYLLRSPFYDRFSEAKILFLLQLLTDHIPGVGLVARPLMDYLPSWQKIYFYSWG
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_145122
• ORF Size: 1008 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145122.1, NP_660104.1
• RefSeq Size: 1221 bp
• RefSeq ORF: 1011 bp
• Locus ID: 18633
• UniProt ID: Q91XC9
• Cytogenetics: 2 E1
• Gene Summary: Required for peroxisome membrane biogenesis. May play a role in early stages of peroxisome assembly. Can recruit other peroxisomal proteins, such as PEX3 and PMP34, to de novo peroxisomes derived from the endoplasmic reticulum (ER). May function as receptor for PEX3 (By similarity).[UniProtKB/Swiss-Prot Function]