B3gat3 (NM_024256) Mouse Tagged ORF Clone

CAT#: MG204972

B3gat3 (tGFP-tagged) - Mouse beta-1,3-glucuronyltransferase 3 (glucuronosyltransferase I) (B3gat3)

Product Images

Circular map for MG204972

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: B3gat3
• Synonyms: 2810405M13Rik
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG204972 representing NM_024256
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGCTGAAGCTGAAGAACGTGTTTCTTGCCTACTTCCTGGTGTCGATCGCCGGCCTCCTCTACGCTC
  TGGTGCAGCTCGGCCAGCCTTGCGACTGCCTCCCTCCGCTTCGAGCTGCAGCTGAGCAGCTTCGGCAGAA
  GGACCTGAGGATATCCCAGTTGCAAGCTGATCTCCGTCGCCCACCCCCTGTCCCAGCCCAGCCCCCTGAA
  CCTGAGGCCCTGCCTACTATCTATGTCATTACCCCCACCTACGCCAGGCTGGTACAAAAGGCAGAGCTGG
  TTCGGCTGTCCCAGACCCTGAGCTTGGTGCCCCGTCTACACTGGCTGCTAGTGGAGGACGCTGAGAGCCC
  TACCCCGCTGGTCTCGGGGCTGTTGGCCGCCTCTGGTCTCCTCTTTACACACCTGGCTGTCCTTACCCCC
  AAGGCTCAACGGCTTAGGGAAGGTGAGCCAGGCTGGGTCCGGCCCCGAGGAGTGGAACAGCGCAATAAGG
  CCCTCGACTGGCTCCGAGGAAAAGGGGGTGCTGTTGGGGGGGAGAAGGATCCACCGCCACCAGGGACCCA
  AGGAGTCGTGTATTTTGCTGACGATGACAACACCTACAGCCGGGAGCTCTTTAAGGAGATGCGTTGGACT
  CGCGGTGTCTCAGTGTGGCCTGTGGGGCTGGTGGGTGGCCTGCGATTTGAAGGTCCTCAGGTACAGGATG
  GCCGCGTTGTGGGTTTCCACACAGCATGGGAACCCAACAGGCCCTTTCCCTTGGACATGGCGGGATTTGC
  GGTTGCCCTGCCCTTGCTATTGGCTAAGCCCAATGCCCAGTTTGATGCTACTGCACCCCGGGGCCACCTG
  GAAAGTAGTCTCCTGAGCCACCTTGTAGATCCCAAGGACCTGGAGCCACGGGCTGCCAATTGTACTCAGG
  TACTGGTATGGCACACCCGGACAGAGAAACCTAAGATGAAGCAGGAGGAGCAGCTACAACGGCAGGGCCA
  GGGCTCAGACCCAGCCATTGAGGTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG204972 representing NM_024256
  Red=Cloning site Green=Tags(s)
  
  MKLKLKNVFLAYFLVSIAGLLYALVQLGQPCDCLPPLRAAAEQLRQKDLRISQLQADLRRPPPVPAQPPE
  PEALPTIYVITPTYARLVQKAELVRLSQTLSLVPRLHWLLVEDAESPTPLVSGLLAASGLLFTHLAVLTP
  KAQRLREGEPGWVRPRGVEQRNKALDWLRGKGGAVGGEKDPPPPGTQGVVYFADDDNTYSRELFKEMRWT
  RGVSVWPVGLVGGLRFEGPQVQDGRVVGFHTAWEPNRPFPLDMAGFAVALPLLLAKPNAQFDATAPRGHL
  ESSLLSHLVDPKDLEPRAANCTQVLVWHTRTEKPKMKQEEQLQRQGQGSDPAIEV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_024256
• ORF Size: 1005 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024256.2, NP_077218.1
• RefSeq Size: 1599 bp
• RefSeq ORF: 1008 bp
• Locus ID: 72727
• UniProt ID: P58158
• Cytogenetics: 19 A
• Gene Summary: Glycosaminoglycans biosynthesis. Involved in forming the linkage tetrasaccharide present in heparan sulfate and chondroitin sulfate. Transfers a glucuronic acid moiety from the uridine diphosphate-glucuronic acid (UDP-GlcUA) to the common linkage region trisaccharide Gal-beta-1,3-Gal-beta-1,4-Xyl covalently bound to a Ser residue at the glycosaminylglycan attachment site of proteoglycans. Can also play a role in the biosynthesis of l2/HNK-1 carbohydrate epitope on glycoproteins. Stimulates 2-phosphoxylose phosphatase activity of PXYLP1 in presence of uridine diphosphate-glucuronic acid (UDP-GlcUA) during completion of linkage region formation.[UniProtKB/Swiss-Prot Function]