Mcl1 (NM_008562) Mouse Tagged ORF Clone

CAT#: MG204868

  • TrueORF®

Mcl1 (tGFP-tagged) - Mouse myeloid cell leukemia sequence 1 (Mcl1)

ORF Plasmid: DDK tGFP

Lentiviral Particles: DDK DDK w/ Puro mGFP mGFP w/ Puro


  "NM_008562" in other vectors (6)

Reconstitution Protocol

USD 650.00

In Stock*

Size
    • 10 ug

Product Images

Frequently bought together (3)
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Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Symbol Mcl1
Synonyms AW556805; Mcl-1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG204868 representing NM_008562
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTTGGCCTGCGGAGAAACGCGGTCATCGGCTTGAACCTGTACTGCGGCGGCGCCAGCCTCGGCGCGG
GCGGCGGTTCTCCGGCAGGGGCGCGCCTGGTGGCCGAGGAGGCCAAGGCGCGGCGCGAGGGGGGAGGGGA
GGCCGCCCTGCTGCCCGGCGCGCGGGTGGTCGCCCGGCCGCCGCCCGTGGGCGCCGAGGACCCCGACGTC
ACCGCGTCGGCCGAAAGGCGGCTGCATAAGTCGCCCGGCCTCCTCGCCGTGCCGCCCGAGGAGATGGCCG
CGTCGGCCGCCGCCGCCATCGTGTCTCCGGAGGAGGAACTGGACGGCTGCGAGCCGGAGGCCATCGGCAA
GCGCCCGGCCGTGCTGCCCCTCCTGGAGCGCGTGAGCGAGGCGGCCAAGAGCTCCGGGGCCGACGGCTCT
CTGCCCTCCACGCCGCCGCCGCCCGAGGAGGAAGAGGACGACCTATACCGCCAGTCGCTGGAGATCATCT
CGCGCTACTTGCGGGAGCAGGCGACCGGCTCCAAGGACTCGAAGCCTCTGGGCGAGGCGGGCGCGGCGGG
CCGGAGAGCGCTGGAGACCCTGCGGCGCGTGGGCGACGGCGTGCAGCGCAACCACGAGACGGCCTTCCAG
GGCATGCTCCGGAAACTGGACATTAAAAACGAAGGCGATGTTAAATCTTTTTCTCGAGTAATGGTCCATG
TTTTCAAAGATGGCGTAACAAACTGGGGCAGGATTGTGACTCTTATTTCTTTCGGTGCCTTTGTGGCCAA
ACACTTAAAGAGCGTAAACCAAGAAAGCTTCATCGAACCATTAGCAGAAACTATCACAGATGTTCTTGTA
AGGACGAAACGGGACTGGCTTGTCAAACAAAGAGGCTGGGATGGGTTTGTGGAGTTCTTCCACGTACAGG
ACCTAGAAGGCGGCATCAGAAATGTGCTGCTGGCTTTTGCGGGTGTTGCTGGAGTAGGGGCTGGTCTGGC
ATATCTAATAAGA


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG204868 representing NM_008562
Red=Cloning site Green=Tags(s)

MFGLRRNAVIGLNLYCGGASLGAGGGSPAGARLVAEEAKARREGGGEAALLPGARVVARPPPVGAEDPDV
TASAERRLHKSPGLLAVPPEEMAASAAAAIVSPEEELDGCEPEAIGKRPAVLPLLERVSEAAKSSGADGS
LPSTPPPPEEEEDDLYRQSLEIISRYLREQATGSKDSKPLGEAGAAGRRALETLRRVGDGVQRNHETAFQ
GMLRKLDIKNEGDVKSFSRVMVHVFKDGVTNWGRIVTLISFGAFVAKHLKSVNQESFIEPLAETITDVLV
RTKRDWLVKQRGWDGFVEFFHVQDLEGGIRNVLLAFAGVAGVGAGLAYLIR

TRTRPLE - GFP Tag - V
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_008562
ORF Size 993 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_008562.3, NP_032588.1
RefSeq Size 3498 bp
RefSeq ORF 996 bp
Locus ID 17210
UniProt ID P97287
Cytogenetics 3 40.74 cM
Gene Summary Involved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. Isoform 2 has antiapoptotic activity.[UniProtKB/Swiss-Prot Function]

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