Sdsl (NM_133902) Mouse Tagged ORF Clone

CAT#: MG204823

Sdsl (tGFP-tagged) - Mouse serine dehydratase-like (Sdsl)

Product Images

Circular map for MG204823

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Sdsl
• Synonyms: 4432411H13Rik; AI504310; SDH1; SDS-RS1; TDH
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG204823 representing NM_133902
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGGGGCCTTGGCAGAACGCGTCGGGGCGGAGCCTTTCCACAGGGTCACGCCCCTGCTGGAGAGCT
  GGGCGCTGTCTCAGGTGGCAGGCATGCCGGTCTTCCTCAAATATGAGAATGTGCAGATAGCTGGCTCCTT
  TAAGATTCGGGGCATCGGACATTTCTGCCAGCAGATGGCCAAGAGGGGATGCAGACATCTGGTGTGCTCC
  TCAGGGGGCAATGCGGGCATTGCGGCTGCATACTCGGCTCGTAAGCTGGGCATCCCCGTCACCATCGTGC
  TCCCAGAGGGCACCTCCGTGCAGGTGGTGAGGCGGCTCGAGGGGGAAGGGGCCGAGGTCCAGCTGACTGG
  GAAAGTCTGGGATGAAGCCAATGTAAAAGCACAAGAACTGGCCACAAGGGATGGCTGGGTGAACGTCTCC
  CCGTTTGACCATCCCCTTATATGGGAAGGCCATGCCAGCCTAGTGCGGGAGCTGAAGGAGTCACTAGGGA
  CCCCTCCAGGTGCTGTGGTGCTGGCCGTGGGGGGCGGAGGGCTCCTGGCAGGTGTGACTGCTGGCCTGCT
  GGAGGTGGGCTGGCAGCATGTGCCCATCGTTGCCATGGAGACCCGCGGGGCGCACAGTTTCAATTCGGCC
  TTGCAGGCAGGCAGGCCGGTCACCCTGCCAGACATCACCAGTGTAGCCAAGAGCCTCGGAGCCAAGACGG
  TGGCTGCACGGACCTTGGAGTGTGCAAAGGAGTGTGAGGTCCTCTCTGAGGTGGTAGAAGACCGGGAGGC
  TGTCAGCGCTGTGCAGAGGTTCCTGGACGATGAGCGCATGCTGGTGGAACCTGCCTGCGGTGCCGCCCTG
  GCCGCCATCTACTCGGGCATCCTGTGGAGGCTCCAGGCTGAGGGCCGCCTGAGTTCTGCCCTAGCTTCCG
  TTGTGGTCATCGTGTGCGGTGGCAACAACATTAGTAGCCAACAGCTTCAGGAGCTGAAAATCCAGCTGGG
  CTGCAGC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG204823 representing NM_133902
  Red=Cloning site Green=Tags(s)
  
  MEGALAERVGAEPFHRVTPLLESWALSQVAGMPVFLKYENVQIAGSFKIRGIGHFCQQMAKRGCRHLVCS
  SGGNAGIAAAYSARKLGIPVTIVLPEGTSVQVVRRLEGEGAEVQLTGKVWDEANVKAQELATRDGWVNVS
  PFDHPLIWEGHASLVRELKESLGTPPGAVVLAVGGGGLLAGVTAGLLEVGWQHVPIVAMETRGAHSFNSA
  LQAGRPVTLPDITSVAKSLGAKTVAARTLECAKECEVLSEVVEDREAVSAVQRFLDDERMLVEPACGAAL
  AAIYSGILWRLQAEGRLSSALASVVVIVCGGNNISSQQLQELKIQLGCS
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_133902
• ORF Size: 987 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_133902.3
• RefSeq Size: 1223 bp
• RefSeq ORF: 990 bp
• Locus ID: 257635
• UniProt ID: Q8R238
• Cytogenetics: 5 F
• Gene Summary: Has low serine dehydratase and threonine dehydratase activity.[UniProtKB/Swiss-Prot Function]