Mafb (NM_010658) Mouse Tagged ORF Clone

CAT#: MG204681

Mafb (tGFP-tagged) - Mouse v-maf musculoaponeurotic fibrosarcoma oncogene family, protein B (avian) (Mafb)

Product Images

Circular map for MG204681

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Mafb
• Synonyms: kr; Kreisler; Krml; Krml1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG204681 representing NM_010658
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGCGGAGCTGAGCATGGGGCAAGAGCTGCCCACCAGCCCGCTGGCCATGGAGTACGTCAACGACT
  TCGACCTTCTCAAGTTCGACGTGAAGAAGGAGCCCCTGGGGCGCGCGGAGCGTCCGGGCCGGCCATGCAC
  ACGCCTGCAGCCTGCTGGCTCGGTGTCGTCCACCCCGCTCAGCACTCCGTGCAGCTCCGTGCCTTCTTCT
  CCCAGCTTCAGTCCGACTGAACCGAAGACCCATCTCGAGGACCTGTACTGGATGGCGAGCAACTACCAGC
  AGATGAACCCCGAGGCACTCAACCTGACGCCCGAGGACGCGGTGGAGGCGCTCATCGGTTCGCACCCAGT
  GCCCCAGCCGCTGCAGAGCTTCGACGGCTTCCGTAGTGCGCACCACCATCACCACCACCACCACCCTCAT
  CCGCACCACGGGTACCCAGGAGCAGGTGTGACTCACGATGACCTGGGCCAGCACGCTCACCCGCACCATC
  ACCATCATCACCAAGCGTCGCCCCCGCCGTCCAGCGCTGCCAGTCCCGCGCAACAGCTACCCACTAGCCA
  CCCGGGGCCGGGACCGCACGCAACAGCCGCGGCGACGGCTGCGGGCGGCAACGGTAGTGTGGAGGACCGC
  TTCTCTGATGACCAGCTGGTGTCCATGTCGGTGCGTGAGCTGAACCGCCACCTGCGGGGCTTCACCAAGG
  ACGAGGTGATCCGCCTGAAGCAGAAGCGGCGGACCCTGAAGAACCGGGGCTACGCCCAGTCGTGCAGGTA
  TAAACGCGTCCAGCAGAAACATCACCTGGAGAACGAGAAGACGCAGCTCATTCAGCAGGTGGAGCAGCTT
  AAGCAGGAGGTGTCCCGGCTGGCCCGCGAGAGAGACGCCTACAAGGTCAAGTGCGAGAAACTCGCCAACT
  CCGGCTTCAGGGAGGCGGGCTCCACCAGCGACAGCCCCTCCTCTCCTGAGTTCTTTCTG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG204681 representing NM_010658
  Red=Cloning site Green=Tags(s)
  
  MAAELSMGQELPTSPLAMEYVNDFDLLKFDVKKEPLGRAERPGRPCTRLQPAGSVSSTPLSTPCSSVPSS
  PSFSPTEPKTHLEDLYWMASNYQQMNPEALNLTPEDAVEALIGSHPVPQPLQSFDGFRSAHHHHHHHHPH
  PHHGYPGAGVTHDDLGQHAHPHHHHHHQASPPPSSAASPAQQLPTSHPGPGPHATAAATAAGGNGSVEDR
  FSDDQLVSMSVRELNRHLRGFTKDEVIRLKQKRRTLKNRGYAQSCRYKRVQQKHHLENEKTQLIQQVEQL
  KQEVSRLARERDAYKVKCEKLANSGFREAGSTSDSPSSPEFFL
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010658
• ORF Size: 969 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010658.3, NP_034788.1
• RefSeq Size: 3389 bp
• RefSeq ORF: 972 bp
• Locus ID: 16658
• UniProt ID: P54841
• Cytogenetics: 2 80.92 cM
• Gene Summary: Acts as a transcriptional activator or repressor. Plays a pivotal role in regulating lineage-specific hematopoiesis by repressing ETS1-mediated transcription of erythroid-specific genes in myeloid cells. Required for monocytic, macrophage, osteoclast, podocyte and islet beta cell differentiation. Involved in renal tubule survival and F4/80 maturation. Activates the insulin and glucagon promoters. Together with PAX6, transactivates weakly the glucagon gene promoter through the G1 element. SUMO modification controls its transcriptional activity and ability to specify macrophage fate. Binds element G1 on the glucagon promoter. Involved either as an oncogene or as a tumor suppressor, depending on the cell context.[UniProtKB/Swiss-Prot Function]