Nhej1 (NM_029342) Mouse Tagged ORF Clone

CAT#: MG204061

Nhej1 (tGFP-tagged) - Mouse nonhomologous end-joining factor 1 (Nhej1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Nhej1
• Synonyms: 1700029B21Rik; cernunnos; XLF
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG204061 representing NM_029342
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGAGCTAGAGCAAGACCTGTTGCTGCAGCCATGGGCATGGTTACAACTTGCGGAGAACTCACTCT
  TAGCCAAGGTGTCTATCACCAAGCACGGTTATGCCTTGCTGATTTCGGATCTTCAACAGGTGTGGCATGA
  ACAGGTGGACACTTCGGTGGTCAGCCAGCGAGCCAAGGAGCTGAACAAGCGCCTCACTGCGCCTCCTGCA
  GCTTTGCTCTGTCACCTGGATGAAGCACTTCGCCCACTGTTTAAAGATTCTGCTCACCCTAGCAAAGCTA
  CTTTCTCCTGTGACCGAGGAGAGGAGGGACTGATCCTGCGGGTGCAGAGTGAGCTCTCGGGTCTTCCCTT
  CAGTTGGCATTTCCACTGTATTCCAGCTAGTTCTTCACTGGTCTCTCAGCATTTGATTCATCCTCTGATG
  GGTGTGAGCCTGGCACTGCAGAGTCATGTGAGGGAGCTAGCAGCATTGCTTCGGATGAAGGACCTTGAGA
  TCCAGGCCTACCAGGAGAGTGGGGCTGTGCTGAGCCGAAGTCGATTGAAGACAGAGCCATTTGAAGAAAA
  TTCTTTCTTGGAACAGTTTATGGCAGAGAAATTGCCAGAGGCGTGTGCTGTTGGTGATGGAAAGCCATTT
  GCCATGAGTCTGCAGAGTCTGTATGTGGCAGTTACAAAACAGCAGATCCAAGCAAGGCAGGCACATAAAG
  ACTCTGGAGAGACTCAGGCATCAAGCAGCACCTCCCCTCGAGGAACTGATAACCAGCCAGAAGAGCCGGT
  CTCCCTCCCTTCCACCCTCTCAGAACCTGAATATGAGCCTGTGGCTGCTTCAGGCCCTATGCATAGAGCT
  CAGCTGGTGAAGTCCAAGAGGAAGAAGCCCAGGGGACTCTTCAGT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG204061 representing NM_029342
  Red=Cloning site Green=Tags(s)
  
  MEELEQDLLLQPWAWLQLAENSLLAKVSITKHGYALLISDLQQVWHEQVDTSVVSQRAKELNKRLTAPPA
  ALLCHLDEALRPLFKDSAHPSKATFSCDRGEEGLILRVQSELSGLPFSWHFHCIPASSSLVSQHLIHPLM
  GVSLALQSHVRELAALLRMKDLEIQAYQESGAVLSRSRLKTEPFEENSFLEQFMAEKLPEACAVGDGKPF
  AMSLQSLYVAVTKQQIQARQAHKDSGETQASSSTSPRGTDNQPEEPVSLPSTLSEPEYEPVAASGPMHRA
  QLVKSKRKKPRGLFS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_029342
• ORF Size: 885 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_029342.2
• RefSeq Size: 1401 bp
• RefSeq ORF: 888 bp
• Locus ID: 75570
• UniProt ID: Q3KNJ2
• Cytogenetics: 1 C4
• Gene Summary: DNA repair protein involved in DNA nonhomologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. May serve as a bridge between XRCC4 and the other NHEJ factors located at DNA ends, or may participate in reconfiguration of the end bound NHEJ factors to allow XRCC4 access to the DNA termini. It may act in concert with XRCC6/XRCC5 (Ku) to stimulate XRCC4-mediated joining of blunt ends and several types of mismatched ends that are noncomplementary or partially complementary (PubMed:17360556). Binds DNA in a length-dependent manner (By similarity).[UniProtKB/Swiss-Prot Function]