Exosc2 (NM_144886) Mouse Tagged ORF Clone

CAT#: MG204028

  • TrueORF®

Exosc2 (tGFP-tagged) - Mouse exosome component 2 (Exosc2)

ORF Plasmid: DDK tGFP

Lentiviral Particles: DDK w/ Puro mGFP w/ Puro

AAV Particle: DDK


  "NM_144886" in other vectors (4)

Reconstitution Protocol

USD 500.00

In Stock*

Size
    • 10 ug

Product Images

Frequently bought together (4)
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Mouse monoclonal turboGFP antibody, clone OTI2H8
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DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
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Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
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Other products for "Exosc2"

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Symbol Exosc2
Synonyms Rrp4
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG204028 representing NM_144886
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGTTGGAGATGAGGCTCCCTAAAGCTCGCAAACCTCTCAGTGAGAGCTTGGGCCGCGACTCTAAGA
AACACTTGGTGGTGCCGGGGGACACGATCACCACGGACACAGGCTTCATGCGGGGCCATGGGACATACAT
GGGGGAAGAGAAGCTCATTGCGTCTGTGGCCGGCTCTGTGGAGAGAGTGAACAAACTGATCTGTGTCAAA
GCCTTGAAAACCAGATACAATGGAGAGGTCGGAGACATTGTAGTGGGGAGAATCACAGAGGTTCAACAGA
AGAGGTGGAAGGTAGAGACCAATTCCAGGCTGGACTCCGTCTTGCTCCTCTCATCCATGAACCTACCTGG
GGGAGAGCTGAGGAGAAGGTCTGCGGAGGATGAGCTGGCCATGAGAGGCTTCTTGCAGGAAGGCGACCTC
ATCAGTGCGGAGGTCCAGGCTGTGTTCTCGGATGGAGCTGTTTCTCTGCACACGAGGAGCCTGAAATATG
GGAAGCTAGGTCAGGGAGTTTTAGTCCAGGTCTCGCCTTCCCTGGTGAAAAGACAGAAGACTCATTTCCA
TGACTTGCCATGTGGTGCCTCAGTGATTCTGGGTAACAACGGTTTCATCTGGATCTACCCAACACCTGAG
CACAAGGATGAGGATGCTGGGGGCTTCATTGCTAACTTGGAGCCCGTAGCTCTTAGTGATCGAGAGGTGA
TCTCCCGGCTTCGGAACTGTGTAGTCTTGCTGGTAACTCAGAGAATGATGCTGTTCGACACCAGCATCCT
GTACTGCTATGAGGCCTCCCTTGCGCATCAGATCAAGGATATCTTAAAACCGGAAGTAATGGAGGAGATC
ATGCTGGAAACACGCCAGAGGCTTTTGGACCAGGAGGGA


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG204028 representing NM_144886
Red=Cloning site Green=Tags(s)

MALEMRLPKARKPLSESLGRDSKKHLVVPGDTITTDTGFMRGHGTYMGEEKLIASVAGSVERVNKLICVK
ALKTRYNGEVGDIVVGRITEVQQKRWKVETNSRLDSVLLLSSMNLPGGELRRRSAEDELAMRGFLQEGDL
ISAEVQAVFSDGAVSLHTRSLKYGKLGQGVLVQVSPSLVKRQKTHFHDLPCGASVILGNNGFIWIYPTPE
HKDEDAGGFIANLEPVALSDREVISRLRNCVVLLVTQRMMLFDTSILYCYEASLAHQIKDILKPEVMEEI
MLETRQRLLDQEG

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_144886
ORF Size 879 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_144886.3
RefSeq Size 1659 bp
RefSeq ORF 882 bp
Locus ID 227715
UniProt ID Q8VBV3
Cytogenetics 2 B
Gene Summary Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes. EXOSC2 as peripheral part of the Exo-9 complex stabilizes the hexameric ring of RNase PH-domain subunits through contacts with EXOSC4 and EXOSC7 (By similarity).[UniProtKB/Swiss-Prot Function]

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