Uck2 (NM_030724) Mouse Tagged ORF Clone

CAT#: MG203431

Uck2 (tGFP-tagged) - Mouse uridine-cytidine kinase 2 (Uck2)

Product Images

Circular map for MG203431

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Uck2
• Synonyms: AA407809; AI481316; AU018180; AU020720; TSA903; UK; UMK; Umpk
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG203431 representing NM_030724
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGGGGACAGCGAGCAGACCCTGCAGAACCACCAGCAGCCCAACGGCGGCGAGCCCTTCCTGATCG
  GCGTGAGCGGGGGCACGGCCAGCGGGAAGTCTTCCGTTTGTGCTAAGATCGTCCAGCTTTTGGGGCAGAA
  TGAGGTGGATTACCACCAGAAGCAGGTGGTGATCCTGAGCCAGGATAGCTTCTACCGAGTCCTCACCTCA
  GAGCAGAAGGCCAAAGCCCTCAAGGGCCAATTCAACTTTGATCACCCGGATGCCTTTGACAACGAACTCA
  TCTTCAAAACACTCAAAGAAATCACCGAAGGAAAAACCGTCCAGATCCCCGTATACGACTTTGTCTCCCA
  CTCACGGAAAGAGGAGACGGTCACCATCTACCCCGCCGACGTGGTGCTCTTCGAAGGGATCCTAGCCTTC
  TATTCCCAGGAGGTCCGAGACCTGTTCCAGATGAAGCTTTTCGTGGACACAGATGCGGATACCCGTCTGT
  CTCGCCGAGTATTGAGGGACATCAGCGAAAGAGGGAGGGACCTTGAGCAGATTTTATCACAGTACATTAC
  GTTTGTGAAGCCTGCCTTTGAGGAATTCTGCTTGCCAACAAAGAAATACGCCGATGTGATCATTCCTAGA
  GGTGCCGACAATCTCGTGGCCATCAACCTCATCGTGCAACACATCCAGGACATCCTCAACGGGGGGCTCT
  CCAAGCGGCAGACGAACGGCTATCTCAACGGCTACACCCCTTCCCGCAAGAGGCAGGCGTCAGAGTCCAG
  CAGCCGACCACAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG203431 representing NM_030724
  Red=Cloning site Green=Tags(s)
  
  MAGDSEQTLQNHQQPNGGEPFLIGVSGGTASGKSSVCAKIVQLLGQNEVDYHQKQVVILSQDSFYRVLTS
  EQKAKALKGQFNFDHPDAFDNELIFKTLKEITEGKTVQIPVYDFVSHSRKEETVTIYPADVVLFEGILAF
  YSQEVRDLFQMKLFVDTDADTRLSRRVLRDISERGRDLEQILSQYITFVKPAFEEFCLPTKKYADVIIPR
  GADNLVAINLIVQHIQDILNGGLSKRQTNGYLNGYTPSRKRQASESSSRPH
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_030724
• ORF Size: 783 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_030724.1, NP_109649.1
• RefSeq Size: 1354 bp
• RefSeq ORF: 786 bp
• Locus ID: 80914
• UniProt ID: Q99PM9
• Cytogenetics: 1 H2.3
• Gene Summary: Phosphorylates uridine and cytidine to uridine monophosphate and cytidine monophosphate. Does not phosphorylate deoxyribonucleosides or purine ribonucleosides. Can use ATP or GTP as a phosphate donor. Can also phosphorylate cytidine and uridine nucleoside analogs such as 6-azauridine, 5-fluorouridine, 4-thiouridine, 5-bromouridine, N(4)-acetylcytidine, N(4)-benzoylcytidine, 5-fluorocytidine, 2-thiocytidine, 5-methylcytidine, and N(4)-anisoylcytidine (By similarity).[UniProtKB/Swiss-Prot Function]