Gstt2 (NM_010361) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG203011
Gstt2 (tGFP-tagged) - Mouse glutathione S-transferase, theta 2 (Gstt2)
  $500.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Gstt2
Synonyms AI266894; mGSTT2; Yrs
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG203011 representing NM_010361
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGCTTGGAGCTCTACCTGGACCTGCTGTCACAACCCAGCCGCGCTGTCTACATCTTCGCCAAGAAGA
ATGGCATCCCCTTCCAGACGCGTACCGTGGATATACTCAAAGGGCAGCACATGAGCGAGCAATTCTCCCA
GGTGAACTGCTTAAACAAAGTTCCTGTACTCAAAGACGGAAGCTTCGTGTTGACCGAAAGCACAGCCATC
TTGATTTACCTGAGTTCCAAGTACCAGGTGGCAGACCACTGGTACCCGGCCGACCTACAGGCCCGTGCCC
AAGTCCACGAATACCTGGGCTGGCATGCCGACAACATCCGTGGTACTTTCGGAGTGCTCCTATGGACCAA
GGTGTTGGGGCCACTCATTGGGGTCCAGGTTCCCCAGGAGAAGGTGGAACGGAACAGAGATAGAATGGTC
CTGGTTCTGCAACAGCTGGAGGACAAGTTCCTCAGGGACAGGGCCTTCCTTGTTGGCCAGCAGGTGACGC
TAGCGGATCTCATGTCTCTGGAGGAGTTGATGCAGCCCGTGGCTCTTGGCTATAACCTGTTTGAGGGACG
GCCTCAGCTGACAGCATGGCGAGAGAGGGTGGAGGCGTTCTTGGGTGCTGAGCTGTGTCAGGAGGCTCAT
AGCACCATCCTGAGCATCCTGGGACAGGCAGCCAAGAAAATGTTACCAGTACCCCCTCCGGAGGTCCATG
CCAGCATGCAGCTTCGAATTGCTAGGATTCCT


AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG203011 representing NM_010361
Red=Cloning site Green=Tags(s)

MGLELYLDLLSQPSRAVYIFAKKNGIPFQTRTVDILKGQHMSEQFSQVNCLNKVPVLKDGSFVLTESTAI
LIYLSSKYQVADHWYPADLQARAQVHEYLGWHADNIRGTFGVLLWTKVLGPLIGVQVPQEKVERNRDRMV
LVLQQLEDKFLRDRAFLVGQQVTLADLMSLEELMQPVALGYNLFEGRPQLTAWRERVEAFLGAELCQEAH
STILSILGQAAKKMLPVPPPEVHASMQLRIARIP

SGPTRTRRLE - GFP Tag - V
Restriction Sites SgfI-RsrII Cloning Scheme for this gene | Plasmid Map
ACCN NM_010361
ORF Size 732 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_010361.2
RefSeq Size 732 bp
RefSeq ORF 735 bp
Locus ID 14872
UniProt ID Q61133
Cytogenetics 10 38.58 cM
Summary Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_010361" in other vectors (4)
SKU Description Size Price
MC203774 Gstt2 (untagged) - Mouse glutathione S-transferase, theta 2 (Gstt2), (10ug) 10 ug
$300.00
MR203011 Gstt2 (Myc-DDK-tagged) - Mouse glutathione S-transferase, theta 2 (Gstt2) 10 ug
$289.00 $300.00
MR203011L3 Lenti ORF clone of Gstt2 (Myc-DDK-tagged) - Mouse glutathione S-transferase, theta 2 (Gstt2) 10 ug
$600.00
MR203011L4 Lenti ORF clone of Gstt2 (mGFP-tagged) - Mouse glutathione S-transferase, theta 2 (Gstt2) 10 ug
$600.00

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