Gch1 (NM_008102) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG202967
Gch1 (tGFP-tagged) - Mouse GTP cyclohydrolase 1 (Gch1)
  $650.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Gch1
Synonyms Gch; GTP-CH; GTPCH
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG202967 representing NM_008102
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGAAGCCGCGGGGAGTCAGGTGCACCAATGGGTTCTCCGAGCGGGAGCTGCCGCGGCCCGGGGCCA
GCCCGCCTGCCGAGAAGTCCCGGCCGCCCGAGGCCAAGGGCGCACAGCCGGCCGACGCCTGGAAGGCAGG
GCGGCACCGCAGCGAGGAGGAAAACCAGGTGAACCTCCCCAAACTGGCGGCTGCTTACTCGTCCATTCTG
CTCTCGCTGGGCGAGGACCCCCAGCGGCAGGGGCTGCTCAAGACGCCCTGGAGGGCGGCCACCGCCATGC
AGTACTTCACCAAGGGATACCAGGAGACCATCTCAGATGTCCTGAATGATGCTATATTTGATGAAGATCA
TGACGAGATGGTGATTGTGAAGGACATAGATATGTTCTCCATGTGTGAGCATCACCTTGTTCCATTTGTA
GGAAGGGTCCATATTGGCTATCTTCCTAACAAGCAAGTCCTTGGTCTCAGTAAACTTGCCAGGATTGTAG
AAATCTACAGTAGACGACTACAAGTTCAAGAGCGCCTCACCAAACAGATTGCGGTGGCCATCACAGAAGC
CTTGCAGCCTGCTGGCGTTGGAGTAGTGATTGAAGCGACACACATGTGCATGGTAATGCGAGGCGTGCAG
AAAATGAACAGCAAGACTGTCACTAGCACCATGCTGGGCGTGTTCCGGGAAGACCCCAAGACTCGGGAGG
AGTTCCTCACACTAATCAGGAGC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG202967 representing NM_008102
Red=Cloning site Green=Tags(s)

MEKPRGVRCTNGFSERELPRPGASPPAEKSRPPEAKGAQPADAWKAGRHRSEEENQVNLPKLAAAYSSIL
LSLGEDPQRQGLLKTPWRAATAMQYFTKGYQETISDVLNDAIFDEDHDEMVIVKDIDMFSMCEHHLVPFV
GRVHIGYLPNKQVLGLSKLARIVEIYSRRLQVQERLTKQIAVAITEALQPAGVGVVIEATHMCMVMRGVQ
KMNSKTVTSTMLGVFREDPKTREEFLTLIRS

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_008102
ORF Size 723 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_008102.2
RefSeq Size 2764 bp
RefSeq ORF 726 bp
Locus ID 14528
UniProt ID Q05915
Cytogenetics 14 24.6 cM
Summary May positively regulate nitric oxide synthesis in endothelial cells. May be involved in dopamine synthesis. May modify pain sensitivity and persistence.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_008102" in other vectors (4)
SKU Description Size Price
MC200761 Gch1 (untagged) - Mouse GTP cyclohydrolase 1 (Gch1), (10ug) 10 ug
$450.00
MR202967 Gch1 (Myc-DDK-tagged) - Mouse GTP cyclohydrolase 1 (Gch1) 10 ug
$450.00
MR202967L3 Lenti ORF clone of Gch1 (Myc-DDK-tagged) - Mouse GTP cyclohydrolase 1 (Gch1) 10 ug
$750.00
MR202967L4 Lenti ORF clone of Gch1 (mGFP-tagged) - Mouse GTP cyclohydrolase 1 (Gch1) 10 ug
$750.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.