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D630039A03Rik (NM_178727) Mouse Tagged ORF Clone

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SKU
MG202879
D630039A03Rik (tGFP-tagged) - Mouse RIKEN cDNA D630039A03 gene (D630039A03Rik)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
  $650.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "D630039A03Rik or NM_178727"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol D630039A03Rik
Synonyms RP23-334A5.4
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG202879 representing NM_178727
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCGGATGAAAGGGTTGGCCTGCCCGTGCCCTGCTCTACCCAAGTTCTTGGAACTGGGGACCTGCTTAA
TGGCTGAGGGTTCCAGGACTCAGGCGCCAGGTCAAGGGCCTCCTGTAAACATCCAGTTTCTTCGTGCCCA
GTATGAAGGTCTGAGGAAACAGCAGAGGACCCAGGCCCACCTAATGGTGTTCCCTAAAGAAGGGACCATG
CCTACTCCGGCTGAATCGATGACCAGTGCTGTTTGGATTAACAAGGAGAGAAGGAGCTCGCTGTCACCAG
AGGAGACAGACTCCGAGGCTGAGGGGATGCTGGAGGAAGGGGACAGAAGCTGCCATCAGGCTTCTGAAAC
TCCATGGCACACATACTTAGAGCTGCATCGCTTGGTTCAAACCTTCCATCTGGAAGCCGGTCACCAAGGG
AACCCCAAGGGATACCTTGTGGAGCCAGAACCAAGGTTCTCACCCGAGGGAGACACTGATGTTTTGAAAA
ACAATCAGAAGACACAGCAGGAAGTCAAAATCCCAGAGGTAGCCCAGTGTCAAAGTCAGGAGGGCTGTGC
CCCACTGCAGGTAGCAGGCTCTAGAGCTCAGTATCTTTCTTCCACAAAGCATTTAAGGTCTGGAAAACCA
GCTTACTATCCATTTCCCCAGAGGAAACCACCCAGGATCTCCCAAGCTGCCCGGAACCTGGGCTTATATG
GCCCACCC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG202879 representing NM_178727
Red=Cloning site Green=Tags(s)

MRMKGLACPCPALPKFLELGTCLMAEGSRTQAPGQGPPVNIQFLRAQYEGLRKQQRTQAHLMVFPKEGTM
PTPAESMTSAVWINKERRSSLSPEETDSEAEGMLEEGDRSCHQASETPWHTYLELHRLVQTFHLEAGHQG
NPKGYLVEPEPRFSPEGDTDVLKNNQKTQQEVKIPEVAQCQSQEGCAPLQVAGSRAQYLSSTKHLRSGKP
AYYPFPQRKPPRISQAARNLGLYGPP

TRTRPLE - GFP Tag - V
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_178727
ORF Size 708 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_178727.2, NP_848842.1
RefSeq Size 2929 bp
RefSeq ORF 711 bp
Locus ID 242484
Cytogenetics 4 B3
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Documents
Resources
"NM_178727" in other vectors (4)
SKU Description Size Price
MC207932 D630039A03Rik (untagged) - Mouse RIKEN cDNA D630039A03 gene (D630039A03Rik), (10ug) 10 ug
$480.00
MR202879 D630039A03Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA D630039A03 gene (D630039A03Rik) 10 ug
$450.00
MR202879L3 Lenti ORF clone of D630039A03Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA D630039A03 gene (D630039A03Rik) 10 ug
$750.00
MR202879L4 Lenti ORF clone of D630039A03Rik (mGFP-tagged) - Mouse RIKEN cDNA D630039A03 gene (D630039A03Rik) 10 ug
$750.00

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