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Nudt7 (BC069843) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG202870
Nudt7 (tGFP-tagged) - Mouse nudix (nucleoside diphosphate linked moiety X)-type motif 7 (cDNA clone MGC:77997 IMAGE:5102473)
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  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Nudt7
Synonyms 1300007B24Rik; 2210404C19Rik
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG202870 representing BC069843
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCGCGACCTTGTGGACTCCCGGAGCCTGTCAGAAACAATCTGATAGATGATGCCAAGGCTCGCCTGA
GAAAGTCTGATGTCGGGACCAGATATTCTCACCTGTCCTCTAATAAATTCTCTGTCCTTGTACCACTGCT
GGCCAGAGGAGGAAAGTTGTATTTGATGTTCACGGTCCGCTCAGACAAGCTGAAAAGGGAACCTGGAGAA
GTCTGCTTCCCTGGAGGAAAGAGGGACCCGGTGGACACAGATGACACAGCCACTGCTCTCCGTGAAGCCC
AGGAGGAGGTGGGGCTGCATCCCCACCAAGTGGAGGTGGTCTCTCACCTGGTGCCATACGTATTTGATAA
TGATGCACTGGTAACCCCCGTAGTGGGTTTTCTAGACCACAACTTCCAGGCCCAACCTAATGCTGATGAA
GTGAAGGAAGTCTTCTTTGTGCCTTTGGACTATTTCCTCCATCCCCAAGTCTACTACCAGAAGCAAATCA
CACAGTCTGGCCGTGATTTCATCATGCATTGCTTCGAGTACAAAGACCCTGAGACTGGTGTGAACTACCT
AATCCAGGGAATGACCTCAAAACTGGCTGTGTTGGTGGCCTTAATTATTTTGGAACAAAGTCCTGCCTTC
AAGATTGATTTTGATCTCCATGACCTGATACCGTCTTGTGAAAGGACCTTCCTTTGGAGATATTCTTTAA
GCAAGTTG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG202870 representing BC069843
Red=Cloning site Green=Tags(s)

MSRPCGLPEPVRNNLIDDAKARLRKSDVGTRYSHLSSNKFSVLVPLLARGGKLYLMFTVRSDKLKREPGE
VCFPGGKRDPVDTDDTATALREAQEEVGLHPHQVEVVSHLVPYVFDNDALVTPVVGFLDHNFQAQPNADE
VKEVFFVPLDYFLHPQVYYQKQITQSGRDFIMHCFEYKDPETGVNYLIQGMTSKLAVLVALIILEQSPAF
KIDFDLHDLIPSCERTFLWRYSLSKL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC069843
ORF Size 708 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC069843, AAH69843
RefSeq Size 1069 bp
RefSeq ORF 710 bp
Locus ID 67528
Cytogenetics 8 E1
Summary Coenzyme A diphosphatase which mediates the cleavage of CoA, CoA esters and oxidized CoA with similar efficiencies, yielding 3',5'-ADP and the corresponding 4'-phosphopantetheine derivative as products. CoA into 3',5'-ADP and 4'-phosphopantetheine. Has no activity toward NDP-sugars, CDP-alcohols, (deoxy)nucleoside 5'-triphosphates, nucleoside 5'-di or monophosphates, diadenosine polyphosphates, NAD, NADH, NADP, NADPH or thymidine-5'-monophospho-p-nitrophenyl ester. May be required to eliminate oxidized CoA from peroxisomes, or regulate CoA and acyl-CoA levels in this organelle in response to metabolic demand. Does not play a role in U8 snoRNA decapping activity. Binds U8 snoRNA.UniProtKB/Swiss-Prot Function
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Documents
Resources
"BC069843" in other vectors (1)
SKU Description Size Price
MC206258 Nudt7 (untagged) - Mouse nudix (nucleoside diphosphate linked moiety X)-type motif 7 (cDNA clone MGC:77997 IMAGE:5102473), (10ug) 10 ug
$300.00

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