Pomc (NM_008895) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG202856
Pomc (tGFP-tagged) - Mouse pro-opiomelanocortin-alpha (Pomc1)
  $500.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Pomc
Synonyms ACT; ACTH; alp; alph; alpha-MSH; alphaMSH; BE; Beta-LPH; beta-M; beta-MSH; Clip; gamma-; Gamma-LPH; gamma-MSH; Npp; PO; Pomc-1; Pomc1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG202856 representing NM_008895
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCGAGATTCTGCTACAGTCGCTCAGGGGCCCTGTTGCTGGCCCTCCTGCTTCAGACCTCCATAGATG
TGTGGAGCTGGTGCCTGGAGAGCAGCCAGTGCCAGGACCTCACCACGGAGAGCAACCTGCTGGCTTGCAT
CCGGGCTTGCAAACTCGACCTCTCGCTGGAGACGCCCGTGTTTCCTGGCAACGGAGATGAACAGCCCCTG
ACTGAAAACCCCCGGAAGTACGTCATGGGTCACTTCCGCTGGGACCGCTTCGGCCCCAGGAACAGCAGCA
GTGCTGGCAGCGCGGCGCAGAGGCGTGCGGAGGAAGAGGCGGTGTGGGGAGATGGCAGTCCAGAGCCGAG
TCCACGCGAGGGCAAGCGCTCCTACTCCATGGAGCACTTCCGCTGGGGCAAGCCGGTGGGCAAGAAACGG
CGCCCGGTGAAGGTGTACCCCAACGTTGCTGAGAACGAGTCGGCGGAGGCCTTTCCCCTAGAGTTCAAGA
GGGAGCTGGAAGGCGAGCGGCCATTAGGCTTGGAGCAGGTCCTGGAGTCCGACGCGGAGAAGGACGACGG
GCCCTACCGGGTGGAGCACTTCCGCTGGAGCAACCCGCCCAAGGACAAGCGTTACGGTGGCTTCATGACC
TCCGAGAAGAGCCAGACGCCCCTGGTGACGCTCTTCAAGAACGCCATCATCAAGAACGCGCACAAGAAGG
GCCAG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG202856 representing NM_008895
Red=Cloning site Green=Tags(s)

MPRFCYSRSGALLLALLLQTSIDVWSWCLESSQCQDLTTESNLLACIRACKLDLSLETPVFPGNGDEQPL
TENPRKYVMGHFRWDRFGPRNSSSAGSAAQRRAEEEAVWGDGSPEPSPREGKRSYSMEHFRWGKPVGKKR
RPVKVYPNVAENESAEAFPLEFKRELEGERPLGLEQVLESDAEKDDGPYRVEHFRWSNPPKDKRYGGFMT
SEKSQTPLVTLFKNAIIKNAHKKGQ

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_008895
ORF Size 705 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_008895.4
RefSeq Size 1009 bp
RefSeq ORF 708 bp
Locus ID 18976
UniProt ID P01193
Cytogenetics 12 1.99 cM
Summary This gene encodes a polypeptide hormone precursor that undergoes extensive, tissue-specific, post-translational processing. Processing yields several biologically active peptides, which are involved in diverse cellular functions, such as energy homeostasis, steroidogenesis, and increased melanin production in melanocytes. In mouse deficiency of this gene is associated with obesity, defects in adrenal development, and altered pigmentation. A pseudogene of this gene is located on chromosome 19. Alternative splicing results in multiple transcript variants. provided by RefSeq, Jun 2013
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Documents
Resources
"NM_008895" in other vectors (4)
SKU Description Size Price
MC209201 Pomc (untagged) - Mouse pro-opiomelanocortin-alpha (Pomc), (10ug) 10 ug
$300.00
MR202856 Pomc (Myc-DDK-tagged) - Mouse pro-opiomelanocortin-alpha (Pomc) 10 ug
$289.00 $300.00
MR202856L3 Lenti ORF clone of Pomc (Myc-DDK-tagged) - Mouse pro-opiomelanocortin-alpha (Pomc) 10 ug
$600.00
MR202856L4 Lenti ORF clone of Pomc (mGFP-tagged) - Mouse pro-opiomelanocortin-alpha (Pomc) 10 ug
$600.00

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