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Uchl3 (NM_016723) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG202766
Uchl3 (tGFP-tagged) - Mouse ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase) (Uchl3)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Uchl3 or NM_016723"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Uchl3
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG202766 representing NM_016723
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGGTCAACGCTGGCTGCCGCTGGAGGCCAACCCTGAGGTCACCAACCAGTTTCTCAAGCAGTTAG
GCCTGCATCCTAACTGGCAGTTTGTTGATGTGTACGGAATGGAGCCTGAACTTCTTAGCATGGTACCAAG
ACCAGTATGCGCAGTGTTACTCCTCTTCCCTATCACAGAAAAGTATGAAGTCTTCAGAACAGAAGAGGAA
GAAAAGATAAAATCTCAAGGACAAGATGTGACATCATCAGTATATTTTATGAAACAAACCATCAGCAATG
CCTGTGGAACGATTGGACTAATCCATGCCATTGCGAACAACAAAGACAAGATGCACTTCGAATCAGGGTC
AACATTGAAAAAGTTCCTGGAGGAGTCTGTATCAATGAGCCCTGAAGAGAGAGCCAAATTCCTGGAGAAC
TATGACGCTATTCGAGTTACTCATGAAACCAGTGCACATGAAGGTCAGACTGAGGCACCAAGTATAGATG
AAAAAGTAGATCTTCATTTTATTGCGTTAGTACATGTAGATGGGCATCTCTATGAATTAGATGGACGGAA
ACCATTTCCAATTAACCATGGGAAAACTAGTGATGAGACGTTGTTAGAGGATGCCATAGAAGTTTGCAAG
AAGTTCATGGAACGCGACCCTGATGAGTTAAGATTTAATGCAATTGCTCTCTCAGCGGCA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG202766 representing NM_016723
Red=Cloning site Green=Tags(s)

MEGQRWLPLEANPEVTNQFLKQLGLHPNWQFVDVYGMEPELLSMVPRPVCAVLLLFPITEKYEVFRTEEE
EKIKSQGQDVTSSVYFMKQTISNACGTIGLIHAIANNKDKMHFESGSTLKKFLEESVSMSPEERAKFLEN
YDAIRVTHETSAHEGQTEAPSIDEKVDLHFIALVHVDGHLYELDGRKPFPINHGKTSDETLLEDAIEVCK
KFMERDPDELRFNAIALSAA

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_016723
ORF Size 690 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_016723.2, NP_057932.2
RefSeq Size 947 bp
RefSeq ORF 693 bp
Locus ID 50933
UniProt ID Q9JKB1
Cytogenetics 14 50.9 cM
Summary Deubiquitinating enzyme (DUB) that controls levels of cellular ubiquitin through processing of ubiquitin precursors and ubiquitinated proteins. Thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. Has a 10-fold preference for Arg and Lys at position P3", and exhibits a preference towards 'Lys-48'-linked ubiquitin chains. Deubiquitinates ENAC in apical compartments, thereby regulating apical membrane recycling. Indirectly increases the phosphorylation of IGFIR, AKT and FOXO1 and promotes insulin-signaling and insulin-induced adipogenesis. Required for stress-response retinal, skeletal muscle and germ cell maintenance. May be involved in working memory. Can hydrolyze UBB(+1), a mutated form of ubiquitin which is not effectively degraded by the proteasome.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_016723" in other vectors (4)
SKU Description Size Price
MC209881 Uchl3 (untagged) - Mouse ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase) (Uchl3), (10ug) 10 ug
$330.00
MR202766 Uchl3 (Myc-DDK-tagged) - Mouse ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase) (Uchl3) 10 ug
$289.00 $300.00
MR202766L3 Lenti ORF clone of Uchl3 (Myc-DDK-tagged) - Mouse ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase) (Uchl3) 10 ug
$600.00
MR202766L4 Lenti ORF clone of Uchl3 (mGFP-tagged) - Mouse ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase) (Uchl3) 10 ug
$600.00

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