Timm50 (BC010303) Mouse Tagged ORF Clone

CAT#: MG202741

Timm50 (tGFP-tagged) - Mouse translocase of inner mitochondrial membrane 50 homolog (yeast) (cDNA clone MGC:11857 IMAGE:3597676)

Product Images

Circular map for MG202741

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Timm50
• Synonyms: 2810403L02Rik; AU015082; TIM50L
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG202741 representing BC010303
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATCATCGAGCCCACCAGTCCCTGCCTTCTCCCAGACCCTCTGCGGGAGCCCTACTACCAGCCACCTT
  ATACACTGGTCCTGGAGCTTACCGGGGTCCTTCTGCACCCAGAGTGGTCGCTGGCCACTGGCTGGAGGTT
  TAAGAAGCGTCCAGGCATCGAGACCTTGTTCCAGCAGCTTGCCCCTCTGTATGAAATAGTCATCTTCACA
  TCAGAGACTGGCATGACTGCATTTCCACTCATTGATAGTGTGGACCCTCACGGTTTCATCTCCTACCGTC
  TGTTCCGGGACGCCACCAGATATATGGAGGGACATCATGTGAAGGACATCTCCTGTCTGAATCGGGACCC
  AGCCCGAGTAGTAGTCGTGGACTGCAAGAAGGAAGCTTTCCGCCTCCAGCCCTTCAACGGTGTTGCCCTG
  CGGCCCTGGGATGGCAACTCAGATGACCGGGTCTTGCTGGACCTGTCTGCCTTCCTCAAGACCATTGCAC
  TGAACCAAGTGGAGGACGTCCGGACGGTGCTGGAGCACTATGCTCTAGAGGATGACCCACTGGAGGCATT
  CAAACAGCGGCAGAGCCGGCTTGAGCAGGAGGAGCAGCAGCGCCTGGCCGAGCTCTCCAAGTCCAACAGG
  CAGGGCCTCTCCTTTGGCTCACTCGCCAGCCGACTGTGGCCTCGCTCCAAGCAGCCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG202741 representing BC010303
  Red=Cloning site Green=Tags(s)
  
  MIIEPTSPCLLPDPLREPYYQPPYTLVLELTGVLLHPEWSLATGWRFKKRPGIETLFQQLAPLYEIVIFT
  SETGMTAFPLIDSVDPHGFISYRLFRDATRYMEGHHVKDISCLNRDPARVVVVDCKKEAFRLQPFNGVAL
  RPWDGNSDDRVLLDLSAFLKTIALNQVEDVRTVLEHYALEDDPLEAFKQRQSRLEQEEQQRLAELSKSNR
  QGLSFGSLASRLWPRSKQP
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC010303
• ORF Size: 689 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC010303, AAH10303
• RefSeq Size: 1487 bp
• RefSeq ORF: 689 bp
• Locus ID: 66525
• Cytogenetics: 7 A3
• Gene Summary: Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane. Has some phosphatase activity in vitro; however such activity may not be relevant in vivo.[UniProtKB/Swiss-Prot Function]