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Rpe (NM_025683) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG202734
Rpe (tGFP-tagged) - Mouse ribulose-5-phosphate-3-epimerase (Rpe)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Rpe
Synonyms 2810429B02Rik; 5730518J08Rik
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG202734 representing NM_025683
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGTCGGGCTGCAAGATCGGCCCGTCCATCCTCAACAGCGACCTGGCCAACCTGGGGGCCGAGTGCC
TGCGCATGCTGGACTCGGGGGCCGACTACCTGCACCTGGATGTAATGGACGGGCATTTTGTTCCCAATAT
CACTTTTGGTCACCCTGTGGTTGAAAGCCTCCGAAAGCAGCTAGGCCAGGACCCTTTCTTTGATATGCAC
ATGATGGTGTCTAGGCCAGAACAGTGGGTAAAACCAATGGCTGTGGCAGGTGCCAATCAATATACCTTTC
ATCTTGAGGCAACTGAGAACCCAGGGGCTTTGATTAAAGACATCCGGGAGAATGGGATGAAGGTTGGCCT
TGCCATCAAACCAGGAACTACAGTTGAATATTTGGCCCCATGGGCTAATCAAATAGATATGGCCTTGGTA
ATGACCGTAGAACCTGGGTTTGGAGGGCAGAAATTCATGGAAGACATGATGCCCAAGGTTCACTGGTTGA
GGACCCAGTTTCCAACTTTGGATATAGAGGTCGATGGCGGAGTAGGTCCTGACACTGTTCAGAAGTGTGC
GGAGGCAGGAGCTAACATGATTGTGTCTGGCAGTGCCATTATGAGGAGTGATGACCCCAGAGCTGTTATC
AACTTACTAAGAAACGTTTGCTCAGAAGCGGCTCAGAAACGTTCCCTTGATCGA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG202734 representing NM_025683
Red=Cloning site Green=Tags(s)

MASGCKIGPSILNSDLANLGAECLRMLDSGADYLHLDVMDGHFVPNITFGHPVVESLRKQLGQDPFFDMH
MMVSRPEQWVKPMAVAGANQYTFHLEATENPGALIKDIRENGMKVGLAIKPGTTVEYLAPWANQIDMALV
MTVEPGFGGQKFMEDMMPKVHWLRTQFPTLDIEVDGGVGPDTVQKCAEAGANMIVSGSAIMRSDDPRAVI
NLLRNVCSEAAQKRSLDR

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_025683
ORF Size 684 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_025683.3
RefSeq Size 2631 bp
RefSeq ORF 687 bp
Locus ID 66646
UniProt ID Q8VEE0
Cytogenetics 1 C3
Summary Catalyzes the reversible epimerization of D-ribulose 5-phosphate to D-xylulose 5-phosphate.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_025683" in other vectors (4)
SKU Description Size Price
MC205425 Rpe (untagged) - Mouse ribulose-5-phosphate-3-epimerase (Rpe), (10ug) 10 ug
$300.00
MR202734 Rpe (Myc-DDK-tagged) - Mouse ribulose-5-phosphate-3-epimerase (Rpe) 10 ug
$289.00 $300.00
MR202734L3 Lenti ORF clone of Rpe (Myc-DDK-tagged) - Mouse ribulose-5-phosphate-3-epimerase (Rpe) 10 ug
$600.00
MR202734L4 Lenti ORF clone of Rpe (mGFP-tagged) - Mouse ribulose-5-phosphate-3-epimerase (Rpe) 10 ug
$600.00

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