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Hes6 (NM_019479) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG202615
Hes6 (tGFP-tagged) - Mouse hairy and enhancer of split 6 (Drosophila) (Hes6)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Hes6 or NM_019479"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Hes6
Synonyms AI326893; bHLHb41
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG202615 representing NM_019479
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTCCGTCCCAGGCGCCCAGCCGGGACCGTGCAGGCCAGGAGGATGAGGACCGCTGGGAAGCACGGG
GGGACCGCAAGGCCCGGAAGCCCTTGGTGGAGAAGAAGCGACGCGCACGGATCAACGAGAGTCTTCAGGA
GCTGCGGCTGCTGCTGGCCGGTACCGAGGTGCAGGCCAAGCTAGAGAACGCCGAGGTTCTGGAGCTGACC
GTGAGGCGCGTGCAGGGCGCGCTGCGAGGCCGGGCGCGCGAGCGGGAGCAGCTGCAGGCTGAAGCAAGCG
AGCGCTTCGCTGCTGGCTACATCCAGTGCATGCATGAGGTGCACACGTTCGTGTCCACGTGCCAAGCCAT
CGATGCCACTGTCTCAGCTGAACTCCTGAACCACCTGCTAGAATCCATGCCACTGCGCGAGGGTAGCAGC
TTTCAGGATCTGCTGGGGGACTCCCTAGCTGGGCTGCCTGGAGGCTCTGGGAGGAGCAGCTGGCCTCCAG
GAGGGTCCCCAGAATCCCCATTGTCCAGTCCCCCAGGTCCCGGGGACGACCTGTGTTCTGACCTAGAGGA
GATCCCAGAGGCTGAACTAAACCGGGTGCCTGCTGAGGGGCCGGATTTGGTGTCTACATCCTTAGGCAGC
CTGACTGCAGCTCGCCGGGCTCAGAGTGTGTGGAGGCCTTGG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG202615 representing NM_019479
Red=Cloning site Green=Tags(s)

MAPSQAPSRDRAGQEDEDRWEARGDRKARKPLVEKKRRARINESLQELRLLLAGTEVQAKLENAEVLELT
VRRVQGALRGRAREREQLQAEASERFAAGYIQCMHEVHTFVSTCQAIDATVSAELLNHLLESMPLREGSS
FQDLLGDSLAGLPGGSGRSSWPPGGSPESPLSSPPGPGDDLCSDLEEIPEAELNRVPAEGPDLVSTSLGS
LTAARRAQSVWRPW

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_019479
ORF Size 672 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_019479.2, NP_062352.1
RefSeq Size 1336 bp
RefSeq ORF 675 bp
Locus ID 55927
UniProt ID Q9JHE6
Cytogenetics 1 D
Summary Does not bind DNA itself but suppresses both HES1-mediated N box-dependent transcriptional repression and binding of HES1 to E box sequences. Also suppresses HES1-mediated inhibition of the heterodimer formed by ASCL1/MASH1 and TCF3/E47, allowing ASCL1 and TCF3 to up-regulate transcription in its presence. Promotes cell differentiation.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_019479" in other vectors (4)
SKU Description Size Price
MC207510 Hes6 (untagged) - Mouse hairy and enhancer of split 6 (Drosophila) (Hes6), (10ug) 10 ug
$300.00
MR202615 Hes6 (Myc-DDK-tagged) - Mouse hairy and enhancer of split 6 (Drosophila) (Hes6) 10 ug
$289.00 $300.00
MR202615L3 Lenti ORF clone of Hes6 (Myc-DDK-tagged) - Mouse hairy and enhancer of split 6 (Drosophila) (Hes6) 10 ug
$600.00
MR202615L4 Lenti ORF clone of Hes6 (mGFP-tagged) - Mouse hairy and enhancer of split 6 (Drosophila) (Hes6) 10 ug
$600.00

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