Chmp3 (NM_025783) Mouse Tagged ORF Clone

CAT#: MG202602

Chmp3 (tGFP-tagged) - Mouse vacuolar protein sorting 24 (yeast) (Vps24)

Product Images

Circular map for MG202602

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Chmp3
• Synonyms: 25.1; 4921505F14Rik; 9130011K15Rik; CGI-49; D6Ertd286e; NEDF; Vps24
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG202602 representing NM_025783
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGACTCTTTGGAAAAACCCAGGAGAAGCCTCCCAAAGAGCTGGTTAATGAATGGTCACTGAAGATCA
  GAAAGGAAATGAGAGTTGTTGACAGGCAAATAAGAGATATCCAAAGAGAAGAAGAGAAAGTAAAACGGTC
  TGTGAAAGATGCAGCCAAGAAGGGCCAAAAGGAAGTCTGTGTGGTTCTGGCCAAGGAGATGATCAGGTCA
  AGGAAAGCCGTGAGCAAGCTCTATGCCTCCAAAGCACACATGAACTCTGTGCTCATGGGCATGAAGAACC
  AACTCGCGGTCCTGCGAGTAGCTGGTTCTCTGCAGAAGAGCACAGAAGTGATGAAGGCAATGCAGAGTCT
  TGTGAAGATCCCAGAAATCCAGGCCACCATGCGGGAGCTGTCCAAAGAGATGATGAAGGCTGGAATCATA
  GAGGAGATGTTAGAGGATACATTTGAAAGCATGGATGATCAAGAAGAAATGGAAGAAGCAGCTGAGATGG
  AGATTGACAGAATCCTCTTTGAAATCACAGCAGGAGCCTTGGGCAAAGCACCCAGTAAAGTAACCGATGC
  CCTTCCTGAGCCTGAACCCGCAGGAGCGATGGCTGCCTCCGAAGAGGGAGAAGAGGAAGAAGATGAAGAG
  GACCTGGAGGCTATGCAGTCACGGCTGGCCACACTCAGAAGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG202602 representing NM_025783
  Red=Cloning site Green=Tags(s)
  
  MGLFGKTQEKPPKELVNEWSLKIRKEMRVVDRQIRDIQREEEKVKRSVKDAAKKGQKEVCVVLAKEMIRS
  RKAVSKLYASKAHMNSVLMGMKNQLAVLRVAGSLQKSTEVMKAMQSLVKIPEIQATMRELSKEMMKAGII
  EEMLEDTFESMDDQEEMEEAAEMEIDRILFEITAGALGKAPSKVTDALPEPEPAGAMAASEEGEEEEDEE
  DLEAMQSRLATLRS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_025783
• ORF Size: 672 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025783.4
• RefSeq Size: 2593 bp
• RefSeq ORF: 675 bp
• Locus ID: 66700
• UniProt ID: Q9CQ10
• Cytogenetics: 6 32.17 cM
• Gene Summary: Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis. ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. Selectively binds to phosphatidylinositol 3,5-bisphosphate PtdIns(3,5)P2 and PtdIns(3,4)P2 in preference to other phosphoinositides tested. Involved in late stages of cytokinesis. Plays a role in endosomal sorting/trafficking of EGF receptor (By similarity).[UniProtKB/Swiss-Prot Function]