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Mif4gd (NM_027162) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG202548
Mif4gd (tGFP-tagged) - Mouse MIF4G domain containing (Mif4gd)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Mif4gd or NM_027162"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Mif4gd
Synonyms 1110014L05Rik; 2310075G12Rik
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG202548 representing NM_027162
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCGAGGCCAGTAGAGATGATTATAAAATCCAGTCTTTTGATGCAGAGACGCAGCAGCTGCTGAAGA
CCGCGCTCAAAGATCCAAGTGCTGTGGACTTAGAGAGAGTAGCCAACGTGATTGTGGACCATTCTCTGCA
GGACTGTGTATTCAGCAAGGAAGCAGGACGCATGTGCTACGCCATCATTCAGGCAGAGAGTAAGCAAGCG
GGCCAGAGCGTCTTCCGCCGTGGGCTCCTCAACCGGCTGCAGAAGGAGTATGATGCGCGGGAGCAGCTCC
GAGCATGCTCCCTGCAGGGCTGGGTCTGCTATGTCACCTTTATCTGCAACATCTTTGACTACCTGAGGGT
GAACAACATGCCCATGATGGCCCTGGTTAACCCTGTCTATGACTGTCTCTTCCAGCTGGCCCAGCCTGAG
AGTCTGAGCAGGGAAGAGGAGGTGGACTGCCTGGTGCTGCAGCTGCACCGGGTCGGGGAGCAGCTGGAGA
AGATGAACGGGCAGCGCATGGATGAGCTCTTTATCCTAATCCGGGACGGCTTCCTGCTCCCGACAGACCT
CAGCTCCCTGGCACGGCTGCTGCTGCTGGAGATGATTGAGTTCCGGGCAGCTGGCTGGAAGACCACTCCT
GCTGCCCACAAGTATTACTACAGTGAGGTCTCTGAC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG202548 representing NM_027162
Red=Cloning site Green=Tags(s)

MSEASRDDYKIQSFDAETQQLLKTALKDPSAVDLERVANVIVDHSLQDCVFSKEAGRMCYAIIQAESKQA
GQSVFRRGLLNRLQKEYDAREQLRACSLQGWVCYVTFICNIFDYLRVNNMPMMALVNPVYDCLFQLAQPE
SLSREEEVDCLVLQLHRVGEQLEKMNGQRMDELFILIRDGFLLPTDLSSLARLLLLEMIEFRAAGWKTTP
AAHKYYYSEVSD

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_027162
ORF Size 666 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_027162.3, NP_081438.3
RefSeq Size 1670 bp
RefSeq ORF 669 bp
Locus ID 69674
UniProt ID Q3UBZ5
Cytogenetics 11 E2
Summary Functions in replication-dependent translation of histone mRNAs which differ from other eukaryotic mRNAs in that they do not end with a poly-A tail but a stem-loop. May participate in circularizing those mRNAs specifically enhancing their translation (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_027162" in other vectors (3)
SKU Description Size Price
MR202548 Mif4gd (Myc-DDK-tagged) - Mouse MIF4G domain containing (Mif4gd), transcript variant 1 10 ug
$289.00 $300.00
MR202548L3 Lenti ORF clone of Mif4gd (Myc-DDK-tagged) - Mouse MIF4G domain containing (Mif4gd), transcript variant 1 10 ug
$600.00
MR202548L4 Lenti ORF clone of Mif4gd (mGFP-tagged) - Mouse MIF4G domain containing (Mif4gd), transcript variant 1 10 ug
$600.00

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