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Mymk (NM_025376) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG202529
Tmem8c (tGFP-tagged) - Mouse RIKEN cDNA 1110002H13 gene (1110002H13Rik)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
$489.00 $500.00
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Other products for "Mymk or NM_025376"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Mymk
Synonyms 1110002H13Rik; AI131587; myomaker; Tmem8c
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_025376
ORF Size 663 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_025376.3
RefSeq Size 1329 bp
RefSeq ORF 666 bp
Locus ID 66139
UniProt ID Q9D1N4
Cytogenetics 2 A3
Summary Myoblast-specific protein that mediates myoblast fusion, an essential step for the formation of multi-nucleated muscle fibers (PubMed:23868259, PubMed:28386024, PubMed:28681861, PubMed:30197239). Actively participates in the membrane fusion reaction by mediating the mixing of cell membrane lipids (hemifusion) upstream of MYMX (PubMed:30197239). Acts independently of MYMX (PubMed:30197239). Involved in skeletal muscle regeneration in response to injury by mediating the fusion of satellite cells, a population of muscle stem cells, with injured myofibers (PubMed:25085416). Also involved in skeletal muscle hypertrophy, probably by mediating the fusion of satellite cells with myofibers (PubMed:28186492).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_025376" in other vectors (6)
SKU Description Size Price
MC203616 Tmem8c (untagged) - Mouse transmembrane protein 8C (Tmem8c), transcript variant 1, (10ug) 10 ug
$300.00
MR202529 Tmem8c (Myc-DDK-tagged) - Mouse transmembrane protein 8C (Tmem8c), transcript variant 1 10 ug
$289.00 $300.00
MR202529L1 Lenti ORF clone of Tmem8c (Myc-DDK-tagged) - Mouse transmembrane protein 8C (Tmem8c), transcript variant 1 10 ug
$600.00
MR202529L2 Lenti ORF clone of Tmem8c (mGFP-tagged) - Mouse transmembrane protein 8C (Tmem8c), transcript variant 1 10 ug
$600.00
MR202529L3 Lenti ORF clone of Tmem8c (Myc-DDK-tagged) - Mouse transmembrane protein 8C (Tmem8c), transcript variant 1 10 ug
$600.00
MR202529L4 Lenti ORF clone of Tmem8c (mGFP-tagged) - Mouse transmembrane protein 8C (Tmem8c), transcript variant 1 10 ug
$600.00

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