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Mettl21a (NM_025964) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG202463
Mettl21a (tGFP-tagged) - Mouse RIKEN cDNA 2310038H17 gene (2310038H17Rik)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Mettl21a
Synonyms 2310038H17Rik; AI464204; Fam119a
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG202463 representing NM_025964
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCCTGGTACCCTATGAGGAGAGCGCGGCAATAGGGCTCCAGAAATTCCATAAGCCTCTCGCCACCT
TCTCTTTTGCGAACCACACCATCCAGATCCGTCAGGACTGGAGGCAACTGGGAGTTGCTGCAGTGGTGTG
GGATGCGGCTGTCGTCCTTTCCATGTATCTGGAGATGGGTGCTGTGGAGCTCAGGGGCTGCTCTGCTGTG
GAGCTGGGTGCTGGCACAGGGCTGGTGGGCATAGTGGCTGCCCTGCCGGGTGCTCAGGTGACTATCACGG
ATCGGAAAGTAGCATTAGAGTTTCTTAAGTCAAACGTTGAAGCCAACTTACCTCCCCATATCCAACCTAA
GGCTGTTGTTAAGGAGCTGACTTGGGGACAAAATTTGGAAAGTTTTTCACCTGGAGAATTTGATCTCATA
CTTGGAGCTGATGTCATATACTTAGAAGATACCTTCACAGACCTTCTTCAAACACTGGGACATCTCTGTA
GCAACAATTCTGTGATTCTTTTAGCTTGCCGAATCCGCTATGAACGGGATAGTAACTTCTTAACAATGCT
GGAGAGGCAATTCACTGTGAGTAAGGTTCACTACGATCCTGAGAAGGACGTACACATTTACAAAGCACAG
AAGAGAAACCAGAGGGAGGACTTG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG202463 representing NM_025964
Red=Cloning site Green=Tags(s)

MALVPYEESAAIGLQKFHKPLATFSFANHTIQIRQDWRQLGVAAVVWDAAVVLSMYLEMGAVELRGCSAV
ELGAGTGLVGIVAALPGAQVTITDRKVALEFLKSNVEANLPPHIQPKAVVKELTWGQNLESFSPGEFDLI
LGADVIYLEDTFTDLLQTLGHLCSNNSVILLACRIRYERDSNFLTMLERQFTVSKVHYDPEKDVHIYKAQ
KRNQREDL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_025964
ORF Size 654 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_025964.2, NP_080240.1
RefSeq Size 2667 bp
RefSeq ORF 657 bp
Locus ID 67099
UniProt ID Q9CQL0
Cytogenetics 1 C2
Summary Protein-lysine methyltransferase that selectively trimethylates residues in heat shock protein 70 (HSP70) family members. Contributes to the in vivo trimethylation of Lys residues in HSPA1 and HSPA8. In vitro methylates 'Lys-561' in HSPA1, 'Lys-564' in HSPA2, 'Lys-585' in HSPA5, 'Lys-563' in HSPA6 and 'Lys-561' in HSPA8 (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_025964" in other vectors (4)
SKU Description Size Price
MC204051 Mettl21a (untagged) - Mouse methyltransferase like 21A (Mettl21a), (10ug) 10 ug
$300.00
MR202463 Mettl21a (Myc-DDK-tagged) - Mouse methyltransferase like 21A (Mettl21a) 10 ug
$289.00 $300.00
MR202463L3 Lenti ORF clone of Mettl21a (Myc-DDK-tagged) - Mouse methyltransferase like 21A (Mettl21a) 10 ug
$600.00
MR202463L4 Lenti ORF clone of Mettl21a (mGFP-tagged) - Mouse methyltransferase like 21A (Mettl21a) 10 ug
$600.00

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