Fam213a (NM_027464) Mouse Tagged ORF Clone

CAT#: MG202457

Fam213a (tGFP-tagged) - Mouse RIKEN cDNA 5730469M10 gene (5730469M10Rik)

Product Images

Circular map for MG202457

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Fam213a
• Synonyms: 5730469M10Rik; Adrx; AU040822; Pamm
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG202457 representing NM_027464
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTATGTGGTCCATCGGTGTGGGAGCTGTGGGGGCCGCTGCTGTGGCACTGCTCCTGGCCAACACAG
  ACATGTTTCTGTCCAAGCCCCGAAAAGCAGCACTGGAATATTTGGAAGACATAGACCTGAAAACACTGGA
  GAAAGAACCAAGGACATTCAAAGCAAAGGAGCTGTGGGAGAAGAACGGGGCTGTGATTATGGCTGTACGC
  AGGCCAGGCTGCTTTCTCTGCCGAGCGGAAGCAGCAGACCTGATGTCCTTGAAGCCCAAGTTGGATGAGC
  TAGGTGTCCCTCTCTATGCGGTGGTGAAGGAGCAGGTGAAGAGAGAAGTGGAAGATTTCCAACCTTACTT
  CAAAGGGGAAATCTTCCTGGATGAAAAGAAAAAATTCTATGGTCCAGAGAGGCGGAAGATGATGTTCATG
  GGCCTCATCCGATTGGGGGTGTGGTATAACTCCTTTCGAGCCTGGAATGGAGGCTTCTCTGGAAACCTAG
  AAGGCGAAGGCTTCATCCTTGGAGGAGTTTTTGTGATAGGATCTGGAAAACAGGGCATCCTTCTTGAGCA
  CCGAGAAAAAGAATTTGGAGACAGAGTGAACCCACTCTCTGTTCTGGAGGCTGTAAAGAAAATCAAGCTA
  CAGACTCCAGCGTCCGGGAGAAGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG202457 representing NM_027464
  Red=Cloning site Green=Tags(s)
  
  MGMWSIGVGAVGAAAVALLLANTDMFLSKPRKAALEYLEDIDLKTLEKEPRTFKAKELWEKNGAVIMAVR
  RPGCFLCRAEAADLMSLKPKLDELGVPLYAVVKEQVKREVEDFQPYFKGEIFLDEKKKFYGPERRKMMFM
  GLIRLGVWYNSFRAWNGGFSGNLEGEGFILGGVFVIGSGKQGILLEHREKEFGDRVNPLSVLEAVKKIKL
  QTPASGRS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_027464
• ORF Size: 654 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_027464.4
• RefSeq Size: 1539 bp
• RefSeq ORF: 657 bp
• Locus ID: 70564
• UniProt ID: Q9CYH2
• Cytogenetics: 14 B
• Gene Summary: Involved in redox regulation of the cell (By similarity). Acts as an antioxidant (By similarity). Inhibits TNFSF11-induced NFKB1 and JUN activation and osteoclast differentiation (By similarity). May affect bone resorption and help to maintain bone mass (By similarity). Acts as a negative regulator of macrophage-mediated inflammation by inhibiting macrophage production of inflammatory cytokines, probably through suppression of the MAPK signaling pathway (PubMed:26438880).[UniProtKB/Swiss-Prot Function]