Gstm4 (NM_026764) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG202448
Gstm4 (tGFP-tagged) - Mouse glutathione S-transferase, mu 4 (Gstm4)
  $500.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Gstm4
Synonyms 1110004G14Rik; Gstb-4; Gstb4; GSTM7-7
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG202448 representing NM_026764
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCTATGACACTGGGTTACTGGGACATCCGTGGGCTAGCTCACGCTATTCGGCTGCTCCTAGAATACA
CAGGCTCAAGCTATGAAGAGAAGAGATACACCATGGGAGACGCTCCTGACTATGACCGAAGCCAGTGGCT
GAGTGAGAAGTTCAAATTGGGCCTGGACTTTCCCAATTTGCCTTACTTGATTGATGGGTCACACAAGATC
ACGCAGAGCAATGCCATCCTGCGCTACATTGCCCGCAAGCACAACCTGTGTGGGGAGACAGAGGAAGAGA
AGATTCGCGTGGACATTTTGGAGAACCAGGCTATGGATGTCTCCAATCAGCTGGCTCGAGTCTGTTACAG
CCCAGACTTTGAGAAACTGAAGGTGGAATACTTGGAGCAGCTCCCTGGAATGGTGAAGCTCTTCTCACAG
TTCCTGGGCCAGCGGACATGGTTTGTTGGTGAAAAGATTACTTTTGTAGATTTCCTGGCTTACGATATCC
TGGACCTGCACCTTATATTCGAACCCACGTGCCTGGACGCCTTCCCAAACCTGAAGGACTTTGTGGCCCG
CTTTGAGGTACTGAAGAGGATCTCTGCTTACATGAAGACCAGCCGCTTCCTCCGAACACCCCTATATACA
AAGGTGGCCACTTGGGGCAATAAG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG202448 representing NM_026764
Red=Cloning site Green=Tags(s)

MPMTLGYWDIRGLAHAIRLLLEYTGSSYEEKRYTMGDAPDYDRSQWLSEKFKLGLDFPNLPYLIDGSHKI
TQSNAILRYIARKHNLCGETEEEKIRVDILENQAMDVSNQLARVCYSPDFEKLKVEYLEQLPGMVKLFSQ
FLGQRTWFVGEKITFVDFLAYDILDLHLIFEPTCLDAFPNLKDFVARFEVLKRISAYMKTSRFLRTPLYT
KVATWGNK

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_026764
ORF Size 654 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_026764.3, NP_081040.1
RefSeq Size 1639 bp
RefSeq ORF 657 bp
Locus ID 14865
UniProt ID Q8R5I6
Cytogenetics 3 F2.3
Summary Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Catalyzes the conjugation of leukotriene A4 with reduced glutathione (GSH) to form leukotriene C4. Can also catalyzes the transfer of a glutathionyl group from glutathione (GSH) to 13(S),14(S)-epoxy-docosahexaenoic acid to form maresin conjugate in tissue regeneration 1 (MCTR1), a bioactive lipid mediator that possess potent anti-inflammatory and proresolving actions.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_026764" in other vectors (5)
SKU Description Size Price
MC203058 Gstm4 (untagged) - Mouse glutathione S-transferase, mu 4 (Gstm4), transcript variant 1, (10ug) 10 ug
$300.00
MG213977 Gstm4 (tGFP-tagged) - Mouse glutathione S-transferase mu 4 (Gstm4) transcript variant 1, (10ug) 10 ug
$530.00
MR213977 Gstm4 (Myc-DDK-tagged) - Mouse glutathione S-transferase, mu 4 (Gstm4), transcript variant 1 10 ug
$330.00
MR213977L3 Lenti ORF clone of Gstm4 (Myc-DDK-tagged) - Mouse glutathione S-transferase, mu 4 (Gstm4), transcript variant 1 10 ug
$630.00
MR213977L4 Lenti ORF clone of Gstm4 (mGFP-tagged) - Mouse glutathione S-transferase, mu 4 (Gstm4), transcript variant 1 10 ug
$630.00

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