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Vti1a (NM_016862) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG202429
Vti1a (tGFP-tagged) - Mouse vesicle transport through interaction with t-SNAREs homolog 1A (yeast) (Vti1a)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Vti1a
Synonyms 1110014F16Rik; 1110018K19Rik; 4921537J05Rik; MVti1; MVti1a; Vti1; Vti1-rp2
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG202429 representing NM_016862
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTTCCGACTTCGAAGGGTACGAGCAGGACTTCGCAGTGCTCACTGCTGAGATCACCAGCAAGATTG
CAAGGGTCCCTCGGCTCCCTCCCGATGAGAAGAAGCAAATGGTTGCCAATGTGGAGAAACAGCTTGAAGA
GGCCAGAGAACTGCTTGAACAGATGGATTTGGAAGTTCGAGAAATTCCACCCCAAAGTCGAGGAATGTAT
AGCAACAGGATGAGAAGCTACAAGCAAGAAATGGGGAAACTGGAAACGGATTTTAAAAGGTCACGGATTG
CCTACAGTGACGAAGTACGGAATGAGCTCCTAGGGGATGCTGGGAATTCCTCCGAGAACCAGAGGGCACA
TCTGCTGGATAACACGGAGAGGCTGGAAAGGTCGTCTCGGAGACTAGAGGCTGGGTACCAGATAGCAGTG
GAGACCGAGCAAATCGGCCAAGAGATGTTGGAAAACCTGAGTCATGACAGAGAAAAGATACAGCGGGCAC
GTGACCGACTCCGGGACGCAGACGCAAACCTGGGGAAGAGCTCTCGGATTCTGACGGGGATGCTGCGAAG
AATCATCCAGAACCGTATCCTGCTCGTCATCTTGGGGATCATCGTGGTCATCGCCATCCTGACGGCCATC
GCGTTTTTCGTCAAAGGACAC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG202429 representing NM_016862
Red=Cloning site Green=Tags(s)

MSSDFEGYEQDFAVLTAEITSKIARVPRLPPDEKKQMVANVEKQLEEARELLEQMDLEVREIPPQSRGMY
SNRMRSYKQEMGKLETDFKRSRIAYSDEVRNELLGDAGNSSENQRAHLLDNTERLERSSRRLEAGYQIAV
ETEQIGQEMLENLSHDREKIQRARDRLRDADANLGKSSRILTGMLRRIIQNRILLVILGIIVVIAILTAI
AFFVKGH

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_016862
ORF Size 651 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_016862.2, NP_058558.1
RefSeq Size 3406 bp
RefSeq ORF 654 bp
Locus ID 53611
UniProt ID O89116
Cytogenetics 19 D2
Summary V-SNARE that mediates vesicle transport pathways through interactions with t-SNAREs on the target membrane. These interactions are proposed to mediate aspects of the specificity of vesicle trafficking and to promote fusion of the lipid bilayers. Involved in vesicular transport from the late endosomes to the trans-Golgi network. Along with VAMP7, involved in an non-conventional RAB1-dependent traffic route to the cell surface used by KCNIP1 and KCND2. May be concerned with increased secretion of cytokines associated with cellular senescence.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_016862" in other vectors (4)
SKU Description Size Price
MC203914 Vti1a (untagged) - Mouse vesicle transport through interaction with t-SNAREs homolog 1A (yeast) (Vti1a), (10ug) 10 ug
$300.00
MR202429 Vti1a (Myc-DDK-tagged) - Mouse vesicle transport through interaction with t-SNAREs homolog 1A (yeast) (Vti1a) 10 ug
$289.00 $300.00
MR202429L3 Lenti ORF clone of Vti1a (Myc-DDK-tagged) - Mouse vesicle transport through interaction with t-SNAREs homolog 1A (yeast) (Vti1a) 10 ug
$600.00
MR202429L4 Lenti ORF clone of Vti1a (mGFP-tagged) - Mouse vesicle transport through interaction with t-SNAREs homolog 1A (yeast) (Vti1a) 10 ug
$600.00

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