2700062C07Rik (NM_026529) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG202407
2700062C07Rik (tGFP-tagged) - Mouse RIKEN cDNA 2700062C07 gene (2700062C07Rik)
  $500.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol 2700062C07Rik
Synonyms AI195775; C87515
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG202407 representing NM_026529
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCGGCAGAAGTATTACATTGAAGCGGCTGCTCGGGGCCTGGTGGGAAGCTGCCCGGGCCAGGCTCGGT
ACCTCCTATGGGCCTACAGTTCGACACACGAGGATAACAGCACTTTCCAGGAAACATGTCCACACTGCTT
CCAATTGTTGGTTCTGGATAACTCTAGAGTGCGCCTCAAACCTAAAGCCAAATTGACACCAAAGATACAG
AAACTTCTTAATCGGGAAGCAAGAAATGATACACTCAGTTTTAAAGAAGCAAAACTGTTGAGAAAGTATA
AAGACTCTACAAGTGTGCTGCTGATTACCTGTAGAACATGCAACAGAACAGTGAGACACCATGGTAAGAG
TAGAAGCTTTCTGTGGGCTCTGAAGAGCAACGCCGCCACTGCTGCCAACAAAGCCAGCCCGAAGACGCCA
AAGAGAACGGCTCCAGGCTCTGCAAACCTCGGTCAGAGTACAAATGGTTCCAAAGGCAAGAGTCCCTCCT
TGACCATCAGAACACCTACATCTGGACAGTCAACACCCATTTGCTCCTCAAGGAATGGGAGCAAAAGAAA
GAAACACTTCTCTCAACTAAAAGCACTGCTTAGTCAGAGTGCATCTGACAAGAACCCAAAGCTGGACTTC
AGGCACTTCTTATCTTCTCTA


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG202407 representing NM_026529
Red=Cloning site Green=Tags(s)

MRQKYYIEAAARGLVGSCPGQARYLLWAYSSTHEDNSTFQETCPHCFQLLVLDNSRVRLKPKAKLTPKIQ
KLLNREARNDTLSFKEAKLLRKYKDSTSVLLITCRTCNRTVRHHGKSRSFLWALKSNAATAANKASPKTP
KRTAPGSANLGQSTNGSKGKSPSLTIRTPTSGQSTPICSSRNGSKRKKHFSQLKALLSQSASDKNPKLDF
RHFLSSL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_026529
ORF Size 651 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_026529.3
RefSeq Size 1097 bp
RefSeq ORF 654 bp
Locus ID 68046
UniProt ID Q5XFZ0
Cytogenetics 18 A2
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Documents
Resources
"NM_026529" in other vectors (4)
SKU Description Size Price
MC203216 2700062C07Rik (untagged) - Mouse RIKEN cDNA 2700062C07 gene (2700062C07Rik), (10ug) 10 ug
$300.00
MR202407 2700062C07Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 2700062C07 gene (2700062C07Rik) 10 ug
$289.00 $300.00
MR202407L3 Lenti ORF clone of 2700062C07Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 2700062C07 gene (2700062C07Rik) 10 ug
$600.00
MR202407L4 Lenti ORF clone of 2700062C07Rik (mGFP-tagged) - Mouse RIKEN cDNA 2700062C07 gene (2700062C07Rik) 10 ug
$600.00

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