OriGene Logo

Eif4e (BC085087) Mouse Tagged ORF Clone

  • TrueORF®
Be the first to review this product
SKU
MG202404
Eif4e (tGFP-tagged) - Mouse eukaryotic translation initiation factor 4E (cDNA clone MGC:103177 IMAGE:6438419)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

Frequently Bought Together (4)

TA150041 Mouse monoclonal turboGFP antibody, clone OTI2H8
100 ul
$447.00
CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
$170.00
PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
100 pmol
$59.00
PS100010 pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
10 ug
$780.00

Other products for "Eif4e or BC085087"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Eif4e
Synonyms Eif4e-ps, eIF-4E
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG202404 representing BC085087
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGACTGTGGAACCGGAAACCACCCCTACCACTAATCCCCCACCTGCAGAAGAGGAAAAAACAGAGT
CTAATCAGGAGGTTGCTAACCCAGAGCACTATATTAAACACCCTCTACAGAACAGGTGGGCACTCTGGTT
TTTTAAAAATGATAAAAGCAAAACTTGGCAAGCAAACCTTCGATTGATCTCTAAGTTTGATACTGTTGAA
GACTTTTGGGCTCTATACAACCATATCCAGTTGTCTAGTAATTTAATGCCTGGCTGTGACTACTCACTTT
TTAAGGACGGGATTGAGCCTATGTGGGAAGATGAGAAAAACAAACGAGGAGGACGGTGGCTGATCACACT
GAACAAGCAGCAGAGACGGAGTGACCTCGATCGCTTCTGGCTAGAGACACTGCTGTGCCTTATTGGAGAA
TCTTTCGATGACTACAGTGATGATGTGTGTGGAGCTGTTGTTAATGTTAGAGCTAAAGGTGATAAGATAG
CAATATGGACTACTGAGTGTGAAAACAGAGATGCAGTCACACACATAGGGAGGGTATACAAGGAAAGGTT
AGGACTTCCTCCGAAGATAGTGATTGGTTATCAGTCCCACGCAGACACAGCTACAAAGAGCGGCTCCACC
ACTAAAAATAGGTTTGTTGTT

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG202404 representing BC085087
Red=Cloning site Green=Tags(s)

MATVEPETTPTTNPPPAEEEKTESNQEVANPEHYIKHPLQNRWALWFFKNDKSKTWQANLRLISKFDTVE
DFWALYNHIQLSSNLMPGCDYSLFKDGIEPMWEDEKNKRGGRWLITLNKQQRRSDLDRFWLETLLCLIGE
SFDDYSDDVCGAVVNVRAKGDKIAIWTTECENRDAVTHIGRVYKERLGLPPKIVIGYQSHADTATKSGST
TKNRFVV

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC085087
ORF Size 651 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC085087, AAH85087
RefSeq Size 2366 bp
RefSeq ORF 653 bp
Locus ID 13684
Cytogenetics 3 64.3 cM
Summary This gene encodes a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5' end of messenger RNAs. The encoded protein aids in translation initiation by recruiting ribosomes to the 5'-cap structure. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. This gene acts as a proto-oncogene, and its expression and activation is associated with transformation and tumorigenesis. It has also been associated with autism spectrum disorders. Consistently, knockout of this gene results in increased translation of neuroligins, postsynaptic proteins linked to autism spectrum disorders. Pseudogenes of this gene are found on other chromosomes. Alternative splicing results in multiple transcript variants. provided by RefSeq, Sep 2015
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Documents
Resources
"BC085087" in other vectors (3)
SKU Description Size Price
MR202404 Eif4e (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 4E (cDNA clone MGC:103177 IMAGE:6438419) 10 ug
$289.00 $300.00
MR202404L3 Lenti ORF clone of Eif4e (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 4E (cDNA clone MGC:103177 IMAGE:6438419) 10 ug
$600.00
MR202404L4 Lenti ORF clone of Eif4e (mGFP-tagged) - Mouse eukaryotic translation initiation factor 4E (cDNA clone MGC:103177 IMAGE:6438419) 10 ug
$600.00

Welcome to OriGene AI!

I'm your AI-powered guide to everything OriGene.
Ask me anything:

  • Product specs
  • Ordering help
  • Technical questions
  • How to find exactly what your experiment needs

Prefer a live agent? Connect with us by clicking the green chat icon, as seen in the image below or email our team at custsupport@origene.com.

Velaro Chat Img

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.