Rab2a (NM_021518) Mouse Tagged ORF Clone

CAT#: MG202321

Rab2a (tGFP-tagged) - Mouse RAB2, member RAS oncogene family (Rab2)

Product Images

Circular map for MG202321

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Rab2a
• Synonyms: 9330148M11Rik; C80220; Rab2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG202321 representing NM_021518
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTACGCCTATCTCTTCAAGTACATCATCATCGGCGACACAGGTGTTGGTAAATCGTGCTTATTGC
  TACAGTTTACAGACAAGAGGTTTCAGCCGGTGCATGACCTCACAATTGGTGTAGAGTTTGGTGCTCGGAT
  GATAACGATTGATGGGAAACAGATAAAACTCCAGATCTGGGATACAGCAGGGCAGGAGTCCTTTCGTTCT
  ATCACACGGTCATATTACAGAGGTGCAGCGGGGGCTTTACTAGTGTATGATATTACAAGGAGAGACACGT
  TCAACCACTTGACAACCTGGTTAGAAGATGCCCGTCAGCATTCCAATTCCAACATGGTCATCATGCTTAT
  TGGAAATAAAAGTGACTTAGAATCTAGGAGAGAAGTGAAAAAGGAAGAAGGTGAAGCTTTTGCACGAGAG
  CATGGACTTATCTTCATGGAAACTTCTGCCAAGACTGCGTCTAATGTAGAAAAGGCATTTATTAACACAG
  CAAAAGAAATTTATGAAAAAATCCAAGAAGGGGTCTTTGACATTAATAATGAGGCAAACGGCATTAAAAT
  TGGCCCTCAGCACGCTGCTACCAACGCATCTCATGGAAGCAACCAAGGCGGACAGCAGGCAGGGGGAGGC
  TGCTGT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG202321 representing NM_021518
  Red=Cloning site Green=Tags(s)
  
  MAYAYLFKYIIIGDTGVGKSCLLLQFTDKRFQPVHDLTIGVEFGARMITIDGKQIKLQIWDTAGQESFRS
  ITRSYYRGAAGALLVYDITRRDTFNHLTTWLEDARQHSNSNMVIMLIGNKSDLESRREVKKEEGEAFARE
  HGLIFMETSAKTASNVEKAFINTAKEIYEKIQEGVFDINNEANGIKIGPQHAATNASHGSNQGGQQAGGG
  CC
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021518
• ORF Size: 636 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021518.2, NP_067493.1
• RefSeq Size: 2058 bp
• RefSeq ORF: 639 bp
• Locus ID: 59021
• UniProt ID: P53994
• Cytogenetics: 4 A1
• Gene Summary: Required for protein transport from the endoplasmic reticulum to the Golgi complex.[UniProtKB/Swiss-Prot Function]