Fgf21 (NM_020013) Mouse Tagged ORF Clone

CAT#: MG202264

Fgf21 (tGFP-tagged) - Mouse fibroblast growth factor 21 (Fgf21)

Product Images

Circular map for MG202264

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Fgf21
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG202264 representing NM_020013
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAATGGATGAGATCTAGAGTTGGGACCCTGGGACTGTGGGTCCGACTGCTGCTGGCTGTCTTCCTGC
  TGGGGGTCTACCAAGCATACCCCATCCCTGACTCCAGCCCCCTCCTCCAGTTTGGGGGTCAAGTCCGGCA
  GAGGTACCTCTACACAGATGACGACCAAGACACTGAAGCCCACCTGGAGATCAGGGAGGATGGAACAGTG
  GTAGGCGCAGCACACCGCAGTCCAGAAAGTCTCCTGGAGCTCAAAGCCTTGAAGCCAGGGGTCATTCAAA
  TCCTGGGTGTCAAAGCCTCTAGGTTTCTTTGCCAACAGCCAGATGGAGCTCTCTATGGATCGCCTCACTT
  TGATCCTGAGGCCTGCAGCTTCAGAGAACTGCTGCTGGAGGACGGTTACAATGTGTACCAGTCTGAAGCC
  CATGGCCTGCCCCTGCGTCTGCCTCAGAAGGACTCCCCAAACCAGGATGCAACATCCTGGGGACCTGTGC
  GCTTCCTGCCCATGCCAGGCCTGCTCCACGAGCCCCAAGACCAAGCAGGATTCCTGCCCCCAGAGCCCCC
  AGATGTGGGCTCCTCTGACCCCCTGAGCATGGTAGAGCCTTTACAGGGCCGAAGCCCCAGCTATGCGTCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG202264 representing NM_020013
  Red=Cloning site Green=Tags(s)
  
  MEWMRSRVGTLGLWVRLLLAVFLLGVYQAYPIPDSSPLLQFGGQVRQRYLYTDDDQDTEAHLEIREDGTV
  VGAAHRSPESLLELKALKPGVIQILGVKASRFLCQQPDGALYGSPHFDPEACSFRELLLEDGYNVYQSEA
  HGLPLRLPQKDSPNQDATSWGPVRFLPMPGLLHEPQDQAGFLPPEPPDVGSSDPLSMVEPLQGRSPSYAS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020013
• ORF Size: 630 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020013.4, NP_064397.1
• RefSeq Size: 947 bp
• RefSeq ORF: 633 bp
• Locus ID: 56636
• UniProt ID: Q9JJN1
• Cytogenetics: 7 B3
• Gene Summary: Stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression). Activity probably requires the presence of KLB.[UniProtKB/Swiss-Prot Function]