Clec4n (NM_020001) Mouse Tagged ORF Clone

CAT#: MG202248

Clec4n (tGFP-tagged) - Mouse C-type lectin domain family 4, member n (Clec4n)

Product Images

Circular map for MG202248

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Clec4n
• Synonyms: Clec6a; Clecsf10; Nkcl
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG202248 representing NM_020001
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGCAGGAAAGACAATCCCAAGGGAAGGGAGTCTGCTGGACCCTGAGACTCTGGTCAGCTGCTGTGA
  TTTCCATGTTACTCTTGAGTACCTGTTTCATTGCGAGCTGTGTGGTGACTTACCAATTTATTATGGACCA
  GCCCAGTAGAAGACTATATGAACTTCACACATACCATTCCAGTCTCACCTGCTTCAGTGAAGGGACTATG
  GTGTCAGAAAAAATGTGGGGATGCTGCCCAAATCACTGGAAGTCATTTGGCTCCAGCTGCTACCTCATTT
  CTACCAAGGAGAACTTCTGGAGCACCAGTGAGCAGAACTGTGTTCAGATGGGGGCTCATCTGGTGGTGAT
  CAATACTGAAGCGGAGCAGAATTTCATCACCCAGCAGCTGAATGAGTCACTTTCTTACTTCCTGGGTCTT
  TCGGATCCACAAGGTAATGGCAAATGGCAATGGATCGATGATACTCCTTTCAGTCAAAATGTCAGGTTCT
  GGCACCCCCATGAACCCAATCTTCCAGAAGAGCGGTGTGTTTCAATAGTTTACTGGAATCCTTCGAAATG
  GGGCTGGAATGATGTTTTCTGTGATAGTAAACACAATTCAATATGTGAAATGAAGAAGATTTACCTA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG202248 representing NM_020001
  Red=Cloning site Green=Tags(s)
  
  MVQERQSQGKGVCWTLRLWSAAVISMLLLSTCFIASCVVTYQFIMDQPSRRLYELHTYHSSLTCFSEGTM
  VSEKMWGCCPNHWKSFGSSCYLISTKENFWSTSEQNCVQMGAHLVVINTEAEQNFITQQLNESLSYFLGL
  SDPQGNGKWQWIDDTPFSQNVRFWHPHEPNLPEERCVSIVYWNPSKWGWNDVFCDSKHNSICEMKKIYL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020001
• ORF Size: 627 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020001.2
• RefSeq Size: 1227 bp
• RefSeq ORF: 630 bp
• Locus ID: 56620
• UniProt ID: Q9JKF4
• Cytogenetics: 6 58.3 cM
• Gene Summary: Binds high-mannose carbohydrates in a Ca(2+)-dependent manner. Functional receptor for alpha-mannans on C.albicans hypheas. Plays an important role in the host defense against C.albicans infection by inducing TH17 cell differentiation. Recognizes also, in a mannose-dependent manner, allergens from house dust mite and fungi, by promoting cysteinyl leukotriene production. Recognizes soluble elements from the eggs of Shistosoma mansoni altering adaptive immune responses. Transduces signals through an Fc receptor gamma chain /FCER1G and Syk-CARD9-NF-kappa-B-dependent pathway.[UniProtKB/Swiss-Prot Function]