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Rhoq (NM_145491) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG202150
Rhoq (tGFP-tagged) - Mouse ras homolog gene family, member Q (Rhoq)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Rhoq
Synonyms Arhq; Tc10; TC10A
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG202150 representing NM_145491
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTCACGGGCCCGGCGCGCTGATGCTGAAGTGCGTGGTGGTCGGCGACGGGGCGGTGGGCAAGACGT
GCCTGCTCATGAGCTATGCCAACGACGCCTTCCCCGAGGAGTACGTGCCCACTGTCTTCGACCACTACGC
AGTCAGCGTCACCGTGGGGGGCAAGCAGTACCTCTTGGGACTCTATGACACAGCTGGACAGGAAGACTAT
GATCGTCTGAGGCCTTTATCTTACCCCATGACTGACGTCTTCCTCATATGCTTCTCCGTGGTAAATCCAG
CCTCGTTTCAAAATGTAAAGGAAGAGTGGGTACCAGAGCTAAAGGAATATGCGCCAAACGTCCCCTTCTT
GTTAATAGGAACTCAGATTGATCTCCGAGATGACCCCAAAACTTTAGCAAGACTGAATGACATGAAAGAA
AAACCTGTCTGTGTGGAACAAGGACAGAAACTAGCAAAAGAGATAGGTGCATGCTGCTATGTGGAGTGTT
CAGCTTTAACCCAGAAGGGTTTGAAGACTGTGTTTGATGAGGCTATCATAGCCATTTTAACTCCAAAGAA
ACACACAGTAAAAAAAAGAATAGGATCAAGATGTATAAACTGTTGTTTGATTACG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG202150 representing NM_145491
Red=Cloning site Green=Tags(s)

MAHGPGALMLKCVVVGDGAVGKTCLLMSYANDAFPEEYVPTVFDHYAVSVTVGGKQYLLGLYDTAGQEDY
DRLRPLSYPMTDVFLICFSVVNPASFQNVKEEWVPELKEYAPNVPFLLIGTQIDLRDDPKTLARLNDMKE
KPVCVEQGQKLAKEIGACCYVECSALTQKGLKTVFDEAIIAILTPKKHTVKKRIGSRCINCCLIT

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_145491
ORF Size 615 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_145491.2, NP_663466.2
RefSeq Size 4159 bp
RefSeq ORF 618 bp
Locus ID 104215
UniProt ID Q8R527
Cytogenetics 17 56.9 cM
Summary Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. May play a role in CFTR trafficking to the plasma membrane. Causes the formation of thin, actin-rich surface projections called filopodia (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_145491" in other vectors (4)
SKU Description Size Price
MC202655 Rhoq (untagged) - Mouse ras homolog gene family, member Q (Rhoq), (10ug) 10 ug
$300.00
MR202150 Rhoq (Myc-DDK-tagged) - Mouse ras homolog gene family, member Q (Rhoq) 10 ug
$289.00 $300.00
MR202150L3 Lenti ORF clone of Rhoq (Myc-DDK-tagged) - Mouse ras homolog gene family, member Q (Rhoq) 10 ug
$600.00
MR202150L4 Lenti ORF clone of Rhoq (mGFP-tagged) - Mouse ras homolog gene family, member Q (Rhoq) 10 ug
$600.00

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